We narrowed to 15,158 results for: pET

1. pET-3a-His-MBP-TEV-VC_Cas1_WT

   Plasmid

   #120364

   Purpose

   Expresses wildtype V-C Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VC_Cas1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-3a-His-MBP-TEV-VD_Cas1_WT

   Plasmid

   #120365

   Purpose

   Expresses wildtype V-D Cas1

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   VD_Cas1
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB4 (human) HA-TAT-tag pET

   Plasmid

   #8526

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Tags

   HA

   Expression

   Bacterial

   Mutation

   replace T7 and HIS tags of pET with an HA tag;

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. HOXB7 (human) HIS-tag pET

   Plasmid

   #8538

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB7 (HOXB7 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

   Plasmid

   #117911

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A & H45Q on eDHFR

   Promoter

   T7

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-45b-NFYC-IDR-mEGFP

   Plasmid

   #185015

   Purpose

   Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   NFYC IDR (Nfyc Mouse)
   

   Expression

   Bacterial

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIV-1 integrase W235F in pET-15b

    Plasmid

    #61681

    Purpose

    HIV-1 integrase W235F in pET-15b

    Depositor

    Robert Craigie

    Article

    Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

    Insert

    HIV-1 integrase W235F
    

    Tags

    His

    Mutation

    W235F

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28a SUMO Nup53xl 162-267

    Plasmid

    #86002

    Purpose

    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Type

    Empty backbone

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. HOXC8 (murine) HIS-tag pET

    Plasmid

    #8567

    Depositor

    Corey Largman

    Insert

    HOXC8 (Hoxc8 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. HOXC6 (human) HIS-tag pET

    Plasmid

    #8565

    Depositor

    Corey Largman

    Insert

    HOXC6 (HOXC6 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    This protein starts at an internal Met initially …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

    Plasmid

    #61939

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

    Plasmid

    #61938

    Purpose

    Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF2) (UCHL5 Human)
    hNFRKB (39-156) (NFRKB Human)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156, residues 2-38 dele…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET30a-ASXL1-HARE-HTH domain

    Plasmid

    #107164

    Purpose

    Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    ASXL1 (Asxl1 Mouse)
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Contains amino acids 2-83

    Promoter

    T7

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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