We narrowed to 4,700 results for: golden gate

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pCVL TAL(Nd154C63) SFFV Xba-Sal linker

Plasmid

#50420

Purpose

pTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Lentiviral and TALEN

Expression

Mammalian

Promoter

SFFV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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SpyCas9-T1048-cpGR2(#4670)

Plasmid

#250780

Purpose

Potent chemically-activated and temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with a cpGR2 insertion behind T1048

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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BCTVΔVSΔREP-RT-pDGB3alpha1

Plasmid

#240727

Purpose

BCTV vector containing the GUS:NPTII repair template for HDR experiments. Lacks the BCTV virion sense ORFs and Rep has been mutated.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

RT

Expression

Plant

Mutation

2 stop codons inserted in Rep

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAV035_mCherry_P2A_fsEGFP_GGsite

Plasmid

#219807

Purpose

Fluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34

Depositor

Alexis Komor

Article

Vasquez et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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(155-bp-DIAL-YB_TATA)-mGL-BGH_pKG2846

Plasmid

#246330

Purpose

155-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pTL51_cHS4-CAG-s36GFPHaloTag-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4

Plasmid

#223542

Purpose

PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-HaloTag, mCherry-3NLS, PAC

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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(263-bp-DIAL-YB_TATA)-mGL-BGH_pKG2847

Plasmid

#246331

Purpose

263-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1751

Plasmid

#246066

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 ori

Depositor

Jennifer Doudna

Article

Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

Insert

Panoptes locus

Expression

Bacterial

Promoter

pJEX promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG1526

Plasmid

#246357

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(loxPcon-TET-DIAL-YB-TATA)-mGL-bGH_pKG3016

Plasmid

#246359

Purpose

loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-TET-DIAL-minCMV)-mGL-bGH_pDA028

Plasmid

#246363

Purpose

203-bp DIAL Reporter Plasmid with minCMV expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-TET-DIAL-minSV40)-mGL-bGH_pDA030

Plasmid

#246364

Purpose

203-bp DIAL Reporter Plasmid with minSV40 expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-TET-DIAL-CMV53)-mGL-bGH_pDA046

Plasmid

#246365

Purpose

203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(203-bp-TET-DIAL-MLP)-mGL-bGH_pDA047

Plasmid

#246366

Purpose

203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_((203-bp-DIAL-YB_TATA)-mGL-BGH)rev_DIV_pKG2948

Plasmid

#246343

Purpose

203-bp DIAL Reporter Lentivirus (with divergent iRFP670) expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Lentiviral and Synthetic Biology

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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