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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,023 results for: golden gate

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Showing: 3841 - 3860 of 5023 results

  1. pHW1177

    Plasmid
    #229872
    Purpose
    gene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Tet-Off[TetR-QFAD]-act-4 3'UTR::SEC
    Expression
    Worm
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229872-plasmid-map-sequence-id-460839

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  2. CROPseq-Puro-2xMS2

    Plasmid
    #212627
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212627-plasmid-map-sequence-id-420278

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  3. CROPseq-Puro-12xMS2

    Plasmid
    #212628
    Purpose
    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212628-plasmid-map-sequence-id-422312

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  4. pJB53

    Plasmid
    #226309
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226309-plasmid-map-sequence-id-449921

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  5. pJJB1559

    Plasmid
    #218590
    Purpose
    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer
    Depositor
    John Dueber
    Article
    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038
    Insert
    GES
    Tags
    ePTS1
    Expression
    Yeast
    Mutation
    Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    218590-plasmid-map-sequence-id-432609

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  9. TL1 alphaDdCBE T2 (DddA6-N)

    Plasmid
    #221995
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221995-plasmid-map-sequence-id-438696

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  10. TC mDdCBE A1

    Plasmid
    #221986
    Purpose
    Expresses monomeric mitochondrial cytosine base editor in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-TC TALE A1 repeats-TALE CTD-DddAtox GSVG-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    DddA: S1326G, G1348S, A1398V, S1418G
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221986-plasmid-map-sequence-id-438487

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  11. TC mDdCBE T2

    Plasmid
    #221987
    Purpose
    Expresses monomeric mitochondrial cytosine base editor in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-TC TALE T2 repeats-TALE CTD-DddAtox GSVG-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    DddA: S1326G, G1348S, A1398V, S1418G
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221987-plasmid-map-sequence-id-438492

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  12. ATP6 alphaDdCBE G2

    Plasmid
    #221959
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A, See depositor comments
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221959-plasmid-map-sequence-id-438714

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  16. pro::EX1-2(intΔNACs):GFP

    Plasmid
    #218570
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218570-plasmid-map-sequence-id-438438

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Showing: 3841 - 3860 of 5023 results