We narrowed to 14,431 results for: pET

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pET51b-His-TEV-SNAP-tag_fast

Plasmid

#167271

Purpose

Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET51b-His-TEV-CLIP-tag

Plasmid

#167274

Purpose

Overexpression of CLIP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Ana GvpC-SpyTag-His in pET28a

Plasmid

#85737

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

Depositor

Mikhail Shapiro

Publication

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (fused to SpyTag and His-tagged)

Tags

His-tag and SpyTag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-SUMO-RNF168ring (1-189)

Plasmid

#63570

Purpose

Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

Depositor

Titia Sixma

Publication

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

RNF168 (RNF168 Human)

Tags

6xHis and SUMO3

Expression

Bacterial

Mutation

Ring domain only (residues 1-189)

Promoter

T7

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-ybbR-HIS-sfGFP-DocI

Plasmid

#58708

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Publication

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

sfGFP-DocI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET41a iGluf

Plasmid

#119833

Purpose

Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluf

Tags

GST

Expression

Bacterial

Mutation

E25D

Promoter

T7

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-dH2A

Plasmid

#105500

Purpose

Expression of Drosophila histone H2A

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2A

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-dH2B

Plasmid

#105501

Purpose

Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2B

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Publication

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Publication

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Publication

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Publication

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS

Plasmid

#85734

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

Depositor

Gregory Poon

Publication

Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

Insert

ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)

Tags

6xHis following thrombin cleavage site LVPR|G

Expression

Bacterial

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Bacterial

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET3a RegIIIg

Plasmid

#64936

Purpose

bacterial expression of processed mouse RegIIIgamma

Depositor

Lora Hooper

Publication

Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

Insert

RegIIIg (Reg3g Mouse)

Expression

Bacterial

Mutation

processed form

Promoter

T7

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
YPet-Lysosomes-20

Plasmid

#56636

Purpose

Localization: Lysosome Membrane, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LAMP1

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
YPet-LaminB1-10

Plasmid

#56635

Purpose

Localization: Nuclear Envelope, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
YPet-Mito-7

Plasmid

#56637

Purpose

Localization: Mitochondria, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

YPet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Publication

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only