We narrowed to 14,915 results for: pET

1. pET28a-Avi-Coh (E154C)-HIS

   Plasmid

   #153447

   Purpose

   E. coli expression of Rc. CohE construct with E154C mutation.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.CohE
   

   Use

   Tags

   6xHis and Avi-tag

   Expression

   Bacterial

   Mutation

   E154C mutation on CohE

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SznF H407A H409A H448A

   Plasmid

   #139566

   Purpose

   expressed SznF H407A H409A H448A in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   SznF H407A H409A H448A
   

   Use

   Tags

   hexahistidine

   Expression

   Bacterial

   Mutation

   changed glutamate 215 to alanine

   Promoter

   T7

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_EGFP-LAMA-S98 (for p24)

   Plasmid

   #130717

   Purpose

   Plasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid protein

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-S98
   

   Use

   Tags

   Hisx10 and StrepTag

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET151-GST-PP-RPN13 (19-132) K97E

   Plasmid

   #125825

   Purpose

   Expresses GST-RPN13 (19-132) K97E in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to glutamate

   Promoter

   T7

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET151-GST-PP-RPN13 (19-132) K83A

   Plasmid

   #125829

   Purpose

   Expresses GST-RPN13 (19-132) K83A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 83 to alanine

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET151-GST-PP-RPN13 (19-132) K97A

   Plasmid

   #125823

   Purpose

   Expresses GST-RPN13 (19-132) K97A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to alanine

   Promoter

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET151-GST-PP-RPN13 (19-132) K97D

   Plasmid

   #125824

   Purpose

   Expresses GST-RPN13 (19-132) K97D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed lysine 97 to aspartate

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET151-GST-PP-RPN13 (19-132) R104A

   Plasmid

   #125826

   Purpose

   Expresses GST-RPN13 (19-132) R104A in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to alanine

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET151-GST-PP-RPN13 (19-132) R104D

   Plasmid

   #125827

   Purpose

   Expresses GST-RPN13 (19-132) R104D in E.coli

   Depositor

   Chris Hill

   Article

   Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

   Insert

   RPN13 (ADRM1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   changed arginine 104 to aspartate

   Promoter

   T7

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET151-GST-PP-RPN13 (19-132) R104E

    Plasmid

    #125828

    Purpose

    Expresses GST-RPN13 (19-132) R104E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to glutamate

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Pet28a His6-HSPB7 (zebrafish) C49S

    Plasmid

    #110071

    Purpose

    Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Cysteine 49 to Serine

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid

    #101240

    Purpose

    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FFSARG peptide, ddFLN4 fingerprint
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a asyn Q79N (TAC-->TAT)

    Plasmid

    #105733

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79N

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pet151D-TOPO-BROX 1-411; K141E

    Plasmid

    #89865

    Purpose

    Expresses full length human BROX with mutation K141E in bacteria; internal ID WISP11-299

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Use

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated lysine 141 to glutamic acid

    Promoter

    T7

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet151D-TOPO-BROX 1-411; L212D

    Plasmid

    #89864

    Purpose

    Expresses full length human BROX with mutation L212D in bacteria; internal ID WISP11-300

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Use

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    mutated leucine 212 to aspartic acid

    Promoter

    T7

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-GFP-PP-RPN2 (918-953)

    Plasmid

    #73743

    Purpose

    Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pET151-GFP-PP-RPN2 (932-953)

    Plasmid

    #73745

    Purpose

    Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pET151-GFP-PP-RPN2 (918-947)

    Plasmid

    #73747

    Purpose

    Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-947 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pET151-GFP-PP-RPN2 (797-953)

    Plasmid

    #74228

    Purpose

    [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pET151-Vps4(101-437) L151D

    Plasmid

    #87736

    Purpose

    Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Leu 151 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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