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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,771 results for: IRES

Showing: 4041 - 4060 of 4771 results

  6. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Article
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    158221-plasmid-map-sequence-id-309022

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  7. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  8. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Article
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Promoter
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171098-plasmid-map-sequence-id-336112

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  12. pLenti PGK mKate2-OGT K842M (Puro)

    Plasmid
    #154291
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    K842M mutation (Catalytic Inactive)
    Promoter
    PGK
    Available since
    Dec. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154291-plasmid-map-sequence-id-332432

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  13. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  15. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Promoter
    Available since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  16. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid
    #154292
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    D554N mutation (Ser/Thr glycosylation-incompetent)
    Promoter
    PGK
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    154292-plasmid-map-sequence-id-332434

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  20. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid
    #154293
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; HCF-…
    Promoter
    PGK
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154293-plasmid-map-sequence-id-307161

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Showing: 4041 - 4060 of 4771 results