We narrowed to 5,023 results for: golden gate

Showing: 4081 - 4100 of 5023 results

Results per page:

Sort results by:

pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG1526

Plasmid

#246357

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiX_((203-bp-DIAL-YB_TATA)-mGL-BGH)rev_DIV_pKG2948

Plasmid

#246343

Purpose

203-bp DIAL Reporter Lentivirus (with divergent iRFP670) expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Lentiviral and Synthetic Biology

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-VP16-ZF37-2A-mCherry-BGH_pKG1501

Plasmid

#246355

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-VP16-ZF37-2A-TagBFP-BGH_pKG3223

Plasmid

#246356

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(79-bp-DIAL-YB_TATA)-mGL-BGH_pKG1523

Plasmid

#246329

Purpose

79-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(nested-DIAL-YB_TATA)-mGL-BGH_pKG2876

Plasmid

#246332

Purpose

nested DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre and VCre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-SV40)-mGL-BGH_pKG3687

Plasmid

#246334

Purpose

203-bp DIAL Reporter Plasmid with SV40 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-minCMV2)-mGL-BGH_pKG3680

Plasmid

#246335

Purpose

203-bp DIAL Reporter Plasmid with minCMV2 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-JB42CAT5)-mGL-BGH_pKG3688

Plasmid

#246336

Purpose

203-bp DIAL Reporter Plasmid with JB42CAT5 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-MLP)-mGL-BGH_pKG3681

Plasmid

#246337

Purpose

203-bp DIAL Reporter Plasmid with MLP expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-CMV53)-mGL-BGH_pKG3682

Plasmid

#246338

Purpose

203-bp DIAL Reporter Plasmid with CMV53 expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(203-bp-DIAL-YB_TATA)-mGL-BGH_pKG1525

Plasmid

#246326

Purpose

203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
(27-bp-DIAL-YB_TATA)-mGL-BGH_pKG1524

Plasmid

#246328

Purpose

27-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa and editable by Cre recombinase

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest_PANCEd_AraC_R2_S170_LOV_P1.1_C3

Plasmid

#242154

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P1.1.3 variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV P1.1.3

Expression

Bacterial

Mutation

AraC: E3K, R146L, A152T, R251L, E281K; AsLOV2: D5…

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFC482

Plasmid

#246439

Purpose

The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

CasE

Tags

NLS

Expression

Mammalian

Mutation

SapI domesticated

Promoter

EF-1a

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV824

Plasmid

#246453

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV823

Plasmid

#246452

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEV746

Plasmid

#246449

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge