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pGP-AAV-GFAP-iGABASnFR2-WPRE

Plasmid

#218871

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Publication

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Has service

AAV1 and AAV5

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

GFAP

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

Plasmid

#203539

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

NLS-mScarlet-2A-mScarlet

Use

AAV

Expression

Mammalian

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV 3xgRNA KO;Rbfox3

Plasmid

#240311

Purpose

KO:NeuN

Depositor

Yuki Ogawa

Insert

KO gRNAs for NeuN

Use

AAV

Mutation

NA

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125234

Purpose

Expresses SPARK oβ2AR transmembrane component in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-lck-smV5-4x6T-WPRE

Plasmid

#196416

Purpose

Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV; Astrocyte-selective

Tags

Lck

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

Plasmid

#196677

Purpose

AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

Depositor

Haining Zhong

Publication

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

PKIα-P2A-mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Publication

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-L10a

Plasmid

#166605

Purpose

Encodes Cre-dependent eGFP-L10a under control of the TRE

Depositor

Martin Myers

Publication

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

eGFP-L10a

Use

AAV

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114099

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Publication

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GfaABC1D BARK D110A p2A mCherry

Plasmid

#117692

Purpose

GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Publication

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

AAV

Tags

HA / FLAG

Mutation

D110A

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-vDIO-mScarlet-IRES-tTA

Plasmid

#118030

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Publication

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-vDIO-mScarlet-IRES-tTA

Use

AAV

Promoter

TRE

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL

Plasmid

#192602

Purpose

An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

SERCaMP

Use

AAV

Promoter

CaMKII

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-AAV-CAG-iGABASnFR2-WPRE

Plasmid

#218883

Purpose

AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Publication

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Use

AAV

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-Mac-GFP

Plasmid

#58852

Purpose

AAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-EF1a-YCNano140

Plasmid

#45852

Depositor

Fritjof Helmchen

Insert

YCNano140

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP713(V256C)-P2A-EGFP

Plasmid

#198071

Purpose

The AAV vector for iRFP713(V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(V256C)

Use

AAV

Tags

P2A-EGFP

Mutation

V256C mutation

Promoter

CMV

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8f

Plasmid

#192615

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

Plasmid

#129532

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

Depositor

Erik Sontheimer

Publication

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIA4
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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