We narrowed to 5,339 results for: pcdna3.1

1. eGFP-RAPH1-F3

   Plasmid

   #194862

   Purpose

   Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 868-1062

   Promoter

   CMV

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP-RAPH1-F2

   Plasmid

   #194861

   Purpose

   Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 535-868

   Promoter

   CMV

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

   Plasmid

   #169597

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9 - VP64 fusion
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

   Plasmid

   #169549

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2308-Tier1-pGL3-PPGK-NLuc_TB

   Plasmid

   #169551

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   pGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2380-Tier1-PhCMV-TM

   Plasmid

   #169485

   Purpose

   Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOME0135

   Plasmid

   #178397

   Purpose

   KSHV Kaposincluster-RevCompl-Syn

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   Kaposincluster-RevCompl-SYN
   

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOME0134

   Plasmid

   #178396

   Purpose

   Expresses KSHV Upstream Mir-SYN

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV Upstream Mir SYN
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOME0133

    Plasmid

    #178394

    Purpose

    Expresses KSHV Downstream Mir-SYN

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV Downstream Mir-SYN
    

    Expression

    Mammalian

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOME0235M

    Plasmid

    #178417

    Purpose

    KSHV Reverse compl Sequence Kaposincluster_RevCompl-KapATGdel_Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    ReverseComplement-Kaposincluster_RevCompl_KapATGdel_Syn
    

    Expression

    Mammalian

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

    Plasmid

    #155308

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfAMA1
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    K546M/I547P/I548Y/I549Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid

    #154859

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Glycine 470 changed to Alanine and Glycine 471 ch…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Opto-GPR78

    Plasmid

    #106033

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR78 (GPR78 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Opto-GPR42

    Plasmid

    #106022

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR42 (GPR42 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF4b K294R

    Plasmid

    #118357

    Purpose

    This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    K294R

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-REDD1 N181-211 (#595)

    Plasmid

    #99939

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA181-211insNAAIRS linker

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-HA-REDD1 N187-207 (#596)

    Plasmid

    #99940

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA187-207insNAAIRS linker

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-REDD1 85-232 (#427)

    Plasmid

    #99922

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 85-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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