Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,023 results for: golden gate

Clear keywords
Showing: 4301 - 4320 of 5023 results

  1. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154066-plasmid-map-sequence-id-312954

    Enlarge

  5. pOPS0384

    Plasmid
    #133234
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133234-plasmid-map-sequence-id-287535

    Enlarge

  6. pOPS0385

    Plasmid
    #133235
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133235-plasmid-map-sequence-id-287549

    Enlarge

  7. pOPS0386

    Plasmid
    #133236
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133236-plasmid-map-sequence-id-287551

    Enlarge

  8. pOPS0383

    Plasmid
    #133233
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133233-plasmid-map-sequence-id-287536

    Enlarge

  9. pOPS0380

    Plasmid
    #133230
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133230-plasmid-map-sequence-id-278559

    Enlarge

  13. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Expression
    Worm
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182356-plasmid-map-sequence-id-361657

    Enlarge

  14. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

    Enlarge

  15. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

    Enlarge

  17. pYSD328

    Plasmid
    #253040
    Purpose
    Encodes the Aga2 signal peptide, anti-ALFA-tag nanobody, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-ALFA-tag nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253040-plasmid-map-sequence-id-508937

    Enlarge

  18. pYSD894

    Plasmid
    #253020
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga2 display anchor in a Met15, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253020-plasmid-map-sequence-id-508728

    Enlarge

  19. pYSD892

    Plasmid
    #253018
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga1 del N181aa anchor in a HIS3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253018-plasmid-map-sequence-id-508727

    Enlarge

  20. pYSD891

    Plasmid
    #253017
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-649 stalk display anchor in a LEU2, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253017-plasmid-map-sequence-id-508726

    Enlarge

Showing: 4301 - 4320 of 5023 results