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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW493_U6-sgRNA-entry

Plasmid

#244823

Purpose

Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 single guide RNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW122_SpG-Cas9_U6-entry

Plasmid

#244819

Purpose

Mammalian expression of SpG Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1011

Plasmid

#239933

Purpose

dCas9 without Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1004

Plasmid

#239926

Purpose

sgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-12 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1005

Plasmid

#239927

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1006

Plasmid

#239928

Purpose

sgRNA compatible with TCTP-SynPro-02 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP994

Plasmid

#239916

Purpose

sgRNA-D compatible with TCTP-SynPro-01, TCTP-SynPro-16 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-D

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1001

Plasmid

#239923

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1002

Plasmid

#239924

Purpose

sgRNA compatible with TCTP-SynPro-09 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP993

Plasmid

#239915

Purpose

sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-C

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP995

Plasmid

#239917

Purpose

sgRNA-E compatible with TCTP-SynPro-04, TCTP-SynPro-06 and CaMV 35S-SynPro-03 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-E

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP996

Plasmid

#239918

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-15 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP997

Plasmid

#239919

Purpose

sgRNA compatible with TCTP-SynPro-05 and CaMV 35S-SynPro-07 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP999

Plasmid

#239921

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP998

Plasmid

#239920

Purpose

sgRNA compatible with TCTP-SynPro-06, TCTP-SynPro-13 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1000

Plasmid

#239922

Purpose

sgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1007

Plasmid

#239929

Purpose

sgRNA compatible with TCTP-SynPro-04, TCTP-SynPro-11 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1008

Plasmid

#239930

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LLP1009

Plasmid

#239931

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge