Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,282 results for: golden gate

Clear keywords
Showing: 4321 - 4340 of 5282 results

  1. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

    Enlarge

  2. pKS1612

    Plasmid
    #239530
    Purpose
    Express CpPT1 membrane protein cargo without mTurqoise2 marker fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-SKL
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239530-plasmid-map-sequence-id-475878

    Enlarge

  3. pKS1138

    Plasmid
    #239499
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239499-plasmid-map-sequence-id-475874

    Enlarge

  4. pKS1428

    Plasmid
    #239513
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239513-plasmid-map-sequence-id-475875

    Enlarge

  5. pJJB1434

    Plasmid
    #239494
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239494-plasmid-map-sequence-id-475871

    Enlarge

  6. pKS1137

    Plasmid
    #239498
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239498-plasmid-map-sequence-id-475872

    Enlarge

  7. pKS1139

    Plasmid
    #239500
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239500-plasmid-map-sequence-id-475873

    Enlarge

  8. pKS1024

    Plasmid
    #239495
    Purpose
    Express peroxisomal RsAS
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS-Venus-ePTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239495-plasmid-map-sequence-id-475870

    Enlarge

  9. pVL1176

    Plasmid
    #239537
    Purpose
    Express Venus as ER marker
    Depositor
    John Dueber
    Insert
    Ost1ss-Venus- HDEL
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239537-plasmid-map-sequence-id-475869

    Enlarge

  10. pKS1135

    Plasmid
    #239497
    Purpose
    Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
    Depositor
    John Dueber
    Insert
    Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239497-plasmid-map-sequence-id-475868

    Enlarge

  11. pPSG803

    Plasmid
    #239533
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YMR206W
    Use
    CRISPR
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239533-plasmid-map-sequence-id-475867

    Enlarge

  12. pKS1169

    Plasmid
    #239503
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239503-plasmid-map-sequence-id-475865

    Enlarge

  13. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

    Enlarge

  14. pKS1025

    Plasmid
    #239496
    Purpose
    Express inactive peroxisomal RsAS as negative control
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    Expression
    Yeast
    Mutation
    H360E
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239496-plasmid-map-sequence-id-475861

    Enlarge

  15. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

    Enlarge

  16. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

    Enlarge

  18. pHW1176

    Plasmid
    #229871
    Purpose
    gene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Publication
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    GFP-C1-tbb-2 3'UTR::SEC
    Tags
    GFP-C
    Expression
    Worm
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229871-plasmid-map-sequence-id-475644

    Enlarge

Showing: 4321 - 4340 of 5282 results