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pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Publication

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
XP1 Xwnt-1

Plasmid

#16990

Depositor

Randall Moon

Publication

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-1 (wnt1.L Frog)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pBS/KS-mId1sp

Plasmid

#16043

Depositor

Robert Benezra

Publication

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id1 (Id1 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 390-742

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-701/+114)-Luc

Plasmid

#13409

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pME-H2B-mCardinal (JDW 1418)

Plasmid

#242553

Purpose

Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCardinal

Use

Gateway Cloning

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Publication

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant pLV-SigmaR1-mNeonGreen

Plasmid

#226569

Purpose

Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)

Depositor

Gia Voeltz

Publication

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Lentiviral

Tags

mNeonGreen

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Publication

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATP11A (ATP11A Human)

Tags

HA

Expression

Mammalian

Mutation

E186Q

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-EGFP-CAAX

Plasmid

#208685

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-v4.1

Plasmid

#204636

Purpose

Expression of AsCas12f v4.1 in mammalian cells

Depositor

Weixin Tang

Publication

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCas12f-v4.1

Tags

NLS

Expression

Mammalian

Mutation

D169K, N199K, N328G, D364R

Promoter

CMV

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-PHPLCd1

Plasmid

#202679

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Publication

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLCD1v2(1-170):PAmCherry1 (PLCD1 Human)

Expression

Mammalian

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mcherry-WIPI2d-cs-TEV-STREP

Plasmid

#178912

Purpose

Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

Depositor

James Hurley

Publication

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WIPI2d

Tags

C term strep and N term mcherry

Expression

Mammalian

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMP71-tCD19-LLO-SIINFEKL

Plasmid

#174598

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes

Depositor

Stanley Riddell

Publication

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

membrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen

Expression

Mammalian

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+mTurquoise2-Golgi

Plasmid

#128425

Purpose

Contains insert allowing in-vivo visualization of Golgi apparatus

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTurquoise2-Golgi

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

Plasmid

#139308

Purpose

N-terminal half of split PI-PLC targeted to plasma membrane

Depositor

Gerry Hammond

Publication

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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myc-Miro1 (lacking TM)

Plasmid

#127613

Purpose

Human Miro1 with the transmembrane deleted

Depositor

Josef Kittler

Publication

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Miro1 (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

Deletion of transmembrane domain

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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