A therapeutic cancer vaccine delivers antigens and adjuvants to lymphoid tissues using genetically modified T cells.
Veatch JR, Singhi N, Srivastava S, Szeto JL, Jesernig B, Stull SM, Fitzgibbon M, Sarvothama M, Yechan-Gunja S, James SE, Riddell SR
J Clin Invest. 2021 Aug 16;131(16):e144195. doi: 10.1172/JCI144195. PubMed Article
Veatch JR, Singhi N, Srivastava S, Szeto JL, Jesernig B, Stull SM, Fitzgibbon M, Sarvothama M, Yechan-Gunja S, James SE, Riddell SR
J Clin Invest. 2021 Aug 16;131(16):e144195. doi: 10.1172/JCI144195. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174595 | pMP71-CD40L | Stably expresses the extracellular and transmembrane domain of murine CD40L |
| 174596 | pMP71-GM-CSF | expresses secreted murine GM-CSF |
| 174597 | pMP71-IFN-B | Expresses secreted murine interferon beta |
| 174598 | pMP71-tCD19-LLO-SIINFEKL | expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopes |
| 174599 | pMP71-super-IL-2 | Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab |
| 174600 | pMP71-Flt3L | Secretes the extracellular domain of murine Flt3L |
| 174601 | pMP71-tCD19-SIINFEKL | expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope |
| 174602 | pMP71-IFN-gamma | Constitutive expression of secreted murine IFN gamma |
| 174603 | pMP71-tCD19-llo-mc38tmg | expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1 |
| 174604 | pMP71-mGFP-llo190-mc38minor | expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1 |
| 174605 | pMP71-mtGFP-llo-lama4-alg8 | expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4 |
| 174606 | pMP71-mIL15-IL12rb1-sushi-il12rb2 | Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags |
| 174607 | pMP71-mGFP-LLO-hPMEL | expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope |
| 174608 | pMP71-mGFP-LLO-mPMEL | expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope |
| 174609 | pMP71-tCD19-mGFP | expresses palmitoylated GFP and extracellular and transmembrane domains of murine CD19 |
| 174610 | PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE | PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19 |