We narrowed to 4,736 results for: donor
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Plasmid#175578PurposeDNA donor for transpositionDepositorInsertMini-Tn, GFP cassette
ExpressionBacterialMutationNOAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor
Plasmid#182685PurposeEncodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.DepositorInsertsExpressionMammalianPromoterCMV and NAAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREST-donor
Plasmid#113807PurposeCRISPR donor plasmid to tag human transcription factor REST with GFPDepositorInsertREST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
UseCRISPRMutationrs4864608Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ2-donor
Plasmid#113810PurposeCRISPR donor plasmid to tag human transcription factor TSHZ2 with GFPDepositorInsertTSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
UseCRISPRAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZFX-donor
Plasmid#112366PurposeCRISPR donor plasmid to tag human transcription factor ZFX with GFPDepositorInsertZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
UseCRISPRAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEAD1-donor
Plasmid#112329PurposeCRISPR donor plasmid to tag human transcription factor TEAD1 with GFPDepositorInsertTEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
UseCRISPRAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJUNB-donor
Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
ExpressionBacterialMutationNOAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLR04_AAVS1_Donor_HLAEB2M
Plasmid#131089PurposeDonor template to knock in HLA-E fused to B2MDepositorInsertHLA-E and B2M with flexible linker
UseCRISPRExpressionMammalianAvailable SinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterEF1αAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgDonor_lentiGuide
Plasmid#184168PurposeLentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.DepositorInsertsgDonor
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbECFP
Plasmid#47917PurposeDonor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertECFP
TagsNLSExpressionInsectPromoterAedes aegypti polyubiquitin (PUb)Available SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
ExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
DH10beta F' DOT sbcC (donor strain)
Bacterial Strain#12082DepositorBacterial ResistanceTetracyclineAvailable SinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed-w+
Plasmid#80801PurposeVector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.DepositorInsertspiggyBac-3xP3-DsRed
GMR-white
UseHigh copy, amp resistancePromoter3xP3 and GMR::hsp70Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
R26-EGFP MMEJ donor vector
Plasmid#137926PurposeR0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based methodDepositorInsertSA-EGFP-bpA with micro-homology arms
UseMouse TargetingAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only