164,909 results

1. p5E-Runx1+23

   Plasmid

   #69602

   Purpose

   Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

   Depositor

   Leonard Zon, Owen Tamplin

   Article

   Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

   Inserts

   Runx1 +23 intronic enhancer (Runx1 Mouse)
   Mouse beta-globin minimal promoter
   

   Use

   5' gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human CRISPR Knockout Pooled Library (H3)

   Pooled Library

   #133914

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome - version H3

   Depositor

   Xiaole Shirley Liu, Myles Brown

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

4. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

   Pooled Library

   #1000000095

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   Eric Lander, David Sabatini

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

5. MAP3K15-TAP

   Plasmid

   #69728

   Purpose

   Express tandem tagged MAP3K15 in cells

   Depositor

   Daniel Liebler

   Insert

   Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
   

   Use

   Tags

   HA-Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2944

   Plasmid

   #144420

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SATB2 (SATB2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

   Plasmid Kit

   #1000000154

   Purpose

   Vectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.

   Depositor

   Jeffrey Essner

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pBT279

   Plasmid

   #122609

   Purpose

   express FERNY-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. RF4

   Bacterial Strain

   #62072

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. TFORF0912

    Plasmid

    #143449

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF831 (ZNF831 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2031

    Plasmid

    #142906

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-AAK1

    Plasmid

    #23844

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    AAK1 (AAK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-ArchT-BFP2-TSERex

    Plasmid

    #123312

    Purpose

    Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    ArchT-BFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1415

    Plasmid

    #143496

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF560 (ZNF560 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF1033

    Plasmid

    #143907

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYT1 (MYT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. esmGFP-C10-pVTCB052

    Plasmid

    #231303

    Purpose

    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

    Depositor

    Alexander Rives

    Insert

    esmGFP-C10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. E. coli BL21 (DE3) ΔEntD

    Bacterial Strain

    #192874

    Purpose

    Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

    Depositor

    Christian Chalut, Christophe Guilhot

    Article

    Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

    Bacterial Resistance

    None

    Species

    Escherichia coli

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

18. pBabe EGFR Del1(T790M)

    Plasmid

    #32072

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Delete E746-A750, T790M

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. FpG5

    Plasmid

    #69443

    Purpose

    lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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