167,433 results

1. OCT4-2A-eGFP-PGK-Puro

   Plasmid

   #31938

   Purpose

   Human OCT4 genomic targeting donor

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKJ0463

   Plasmid

   #228715

   Purpose

   Bacterial expression of epT7 RNAP; for protein production and purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Science 0, eadr6006

   Insert

   Sumo_epT7
   

   Expression

   Bacterial

   Mutation

   T3M/G47A/E643G

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEM705 Gs

   Plasmid

   #109350

   Purpose

   Wildtype Gαs

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   GαS (GNAS Human)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-KIF2C

   Plasmid

   #205996

   Purpose

   inducible expression of HA-KIF2C

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   KIF2c (KIF2C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pTight

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY2olig-TDP-43

   Plasmid

   #218920

   Purpose

   Expression of CRY2olig-mCherry fused to TDP-43

   Depositor

   Tatyana Shelkovnikova

   Insert

   CRY2olig-mCherry tagged TDP-43
   

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR196

   Plasmid

   #36029

   Depositor

   Wolf Frommer, Dominique Loque, Doris Rentsch

   Article

   Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV2_HLF-ZE

   Plasmid

   #175034

   Purpose

   HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

   Depositor

   Guy Sauvageau

   Article

   Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

   Insert

   HLF ires ZsGreen P2A tEGFR
   

   Use

   AAV

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM200

   Plasmid

   #13079

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   Ptac-gam-bet-exo operon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR v2 mBAX

   Plasmid

   #129577

   Purpose

   CRISPR deletion of murine BAX

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs murine BAX (Bax Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas9-HE

    Plasmid

    #109400

    Purpose

    Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    HDR-Enhancer domain of human CtIP protein (RBBP8 Human)
    

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0035 Ef1a-Nm60-2a-EGFP

    Plasmid

    #193464

    Purpose

    Expression of Nm60 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-A11(2-88)-Strep

    Plasmid

    #196214

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInt_attP1_sacB_kanR

    Plasmid

    #205301

    Purpose

    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LaM8-AK74-irFP

    Plasmid

    #159592

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Lentiviral

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPR v2 mBAK

    Plasmid

    #129578

    Purpose

    CRISPR deletion of murine BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAK (Bak1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Apex2-Venus-CAAX

    Plasmid

    #168511

    Purpose

    Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

    Insert

    Apex2
    

    Tags

    Venus-CAAX

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.2/V5-DEST RBM14-V5

    Plasmid

    #69823

    Purpose

    Expresses RBM14-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    RNA-binding protein 14 (RBM14 Human)
    

    Use

    Retroviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHelper_TS_V2_ampR

    Plasmid

    #214467

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Promoter

    XylS / AraC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF0432 Ef1a-Dn29-2a-EGFP

    Plasmid

    #193459

    Purpose

    Expression of Dn29 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-GFP1-10-HygR

    Plasmid

    #223675

    Purpose

    To check the split GFP signals in mitochondria (1)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP1-10
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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