169,069 results

1. pXPR_016

   Plasmid

   #107143

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   David Root

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Tet-On-HDAC1-shRNA1

   Plasmid

   #90242

   Purpose

   Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-IRAK2

   Plasmid

   #23412

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   IRAK2 (IRAK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-M-Flag-BAP1-H169Q

   Plasmid

   #125847

   Purpose

   express mutant BAP1-H169Q

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   H169Q

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVHD

   Plasmid

   #61303

   Purpose

   Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag

   Depositor

   Julia Vorholt

   Article

   Kaczmarczyk et al Sci Rep. 2014 Sep 29;4:6453. doi: 10.103

   Type

   Empty backbone

   Tags

   HA

   Expression

   Bacterial

   Promoter

   PV10

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. HTR2A-DuET

   Plasmid

   #213321

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGlomyc-Klf4

   Plasmid

   #172870

   Purpose

   Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Klf4 (Klf4 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

   Plasmid

   #51255

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248 mutated to Ala

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pEBTetD-β-tubulin_SNAP

   Plasmid

   #136832

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   TUBB_SNAP (TUBB Synthetic, Human)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid

    #90243

    Purpose

    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXRA2

    Plasmid

    #63144

    Purpose

    ADE2 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.

    Depositor

    Marc Gartenberg

    Article

    Chou et al FEMS Yeast Res. 2015 May;15(3):fov010. d

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial and Yeast

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pEBTet-SNAP-γ-tubulin

    Plasmid

    #136799

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-TUBG (TUBG1 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBTetD-SNAP-Centrin2

    Plasmid

    #136785

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin2 (CETN2 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM-AAV-hSncg-EGFP

    Plasmid

    #153198

    Purpose

    Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBTet-SNAP-HsSAS6

    Plasmid

    #136789

    Purpose

    Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-SAS6 (SASS6 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEBTet-SNAP-Centrin2

    Plasmid

    #136784

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin2 (CETN2 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-shEsr1

    Plasmid

    #120720

    Purpose

    Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

    Depositor

    Amy Lasek

    Article

    Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Mouse U6

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSN16

    Plasmid

    #100103

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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