164,909 results

1. pCMV_nLightG

   Plasmid

   #217656

   Purpose

   Expresses norepinephrine indicator nLightG under mammalian promoter

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   nLightG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JD638

   Plasmid

   #160395

   Purpose

   Vitis miR396-resistant GRF4-GIF1 in pDONR-zeo

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   Vitis miR396-resistant GRF4-GIF1 chimera
   

   Use

   Entry clon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-SpCas9-NG

   Plasmid

   #171370

   Purpose

   SpCas9-NG-mediated genome editing.

   Depositor

   Masahito Ikawa

   Article

   Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA; CBh for Cas9-2A-puroR

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-UAS-anti-CD3/anti-CD19 BiTE

   Plasmid

   #230005

   Purpose

   Gal4-dependent expression of secreted BiTE

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   UAS-anti-CD3/anti-CD19 BiTE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UAS

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-TRIM28deltaRING

   Plasmid

   #124952

   Purpose

   Mammalian expression of Human TRIM28 with deleted RING domain

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of RING domain

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

   Plasmid

   #155125

   Purpose

   Tol2 vector containing Cre-inducible QFGal4-switch

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QFGal4-switch
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Ubi

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMKLR1-Tango

   Plasmid

   #66253

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CMKLR1 (CMKLR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-ATXN1-2Q

   Plasmid

   #33239

   Depositor

   Huda Zoghbi

   Article

   Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   insert contains 2Q (2 glutamines) and there isno …

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0623

   Plasmid

   #142666

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DDIT3 (DDIT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRIM28 gRNA (BRDN0001146162)

    Plasmid

    #76142

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNL(CMV)TK2ALuc2/CMV/WPREdU3

    Plasmid

    #163965

    Purpose

    Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

    Insert

    HSV-1 TK and FLuc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHCMV-SS-antiEEscFv-TM

    Plasmid

    #198304

    Purpose

    Displays anti-EE scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiEEscFv-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Use

    Tags

    StayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid

    #191500

    Purpose

    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol2 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    myo6b

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTSara

    Plasmid

    #60720

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T7 RNAP split between positions 179-180 and wt T7…

    Promoter

    PBAD

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN1-OPA1(123Nt)-mScarletI

    Plasmid

    #129343

    Purpose

    Mitochondrial intermembrane space localization

    Depositor

    Yasushi Okada

    Insert

    mScarlet(i) (Addgene 85044)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MC5R-Tango

    Plasmid

    #66431

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC5R (MC5R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pVW296

    Plasmid

    #139496

    Purpose

    Expression vector for the PP7-mCherry protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 PP7∆FG-mCherry tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-ChREBPα

    Plasmid

    #211754

    Purpose

    Cloning vector for the expression of mouse ChREBPα

    Depositor

    Russell Goodman

    Article

    Singh et al Cell Metab. 2024 Jan 2;36(1):144-158.e7.

    Insert

    MLXIPL (Mlxipl Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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