163,387 results

1. pBabe puro IRES-EGFP

   Plasmid

   #14430

   Depositor

   L. Miguel Martins

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12315 - pAAV-AiE0464m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2315)

   Plasmid

   #223981

   Purpose

   AiE0464m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e-NG

   Plasmid

   #226856

   Purpose

   Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8e-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT3GEPIR-WEE1shRNA1

   Plasmid

   #220571

   Purpose

   To inducibly knockdown WEE1 expression

   Depositor

   Sean Lee

   Insert

   WEE1 shRNA1 (WEE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LT3GEPIR-WEE1shRNA2

   Plasmid

   #220572

   Purpose

   To inducibly knockdown WEE1 expression

   Depositor

   Sean Lee

   Insert

   WEE1 shRNA2 (WEE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p31sheLL

   Plasmid

   #37322

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV31L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti TurboRFP BlastR

   Plasmid

   #102343

   Purpose

   Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   TurboRFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV10/3xflag-Clock

    Plasmid

    #47334

    Purpose

    full length clock in pCMV10 with 3x flag at the N-terminus

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    clock
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAp63alpha-FLAG

    Plasmid

    #27008

    Depositor

    David Sidransky

    Insert

    TAp63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. All-in-one lenti-evoCDA-NG

    Plasmid

    #200449

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha and mU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)

    Viral Prep

    #55645-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    Syn

    Tags

    EYFP (Cre- and Flp-dependent)

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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