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167,909 results

Showing: 381 - 400 of 167909 results

  1. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  5. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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  6. pFV2-HA

    Plasmid
    #187082
    Purpose
    Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)
    Depositor
    Steven Vik
    Article
    Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1
    Insert
    atpBEFHAGDC
    Tags
    6-His beta subunit (atpD) and HA tag subunit a (a…
    Expression
    Bacterial
    Mutation
    All Cys codons changed to Ala.
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    187082-plasmid-map-sequence-id-372620

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  7. pAAV-CAG-fDIO-mNeonGreen (AAV1)

    Viral Prep
    #99133-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-fDIO-mNeonGreen (#99133). In addition to the viral particles, you will also receive purified pAAV-CAG-fDIO-mNeonGreen plasmid DNA. CAG-driven, Flp-dependent mNeonGreen expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    CAG
    Tags
    mNeonGreen (Flp-dependent)
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-S5E2-ChR2-mCherry (AAV9)

    Viral Prep
    #135634-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    mCherry
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. pGENT R2 GW R1 RUBY

    Plasmid
    #225988
    Purpose
    To express RUBY transgene under the control of desired promoter (Note: R2 Gateway R1)
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Tags
    RUBY
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225988-plasmid-map-sequence-id-448011

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  15. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  20. HPSE-bio-His

    Plasmid
    #53407
    Purpose
    Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    HPSE (HPSE Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53407-plasmid-map-sequence-id-83186

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Showing: 381 - 400 of 167909 results