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pE-SUMO CRYBA1

Plasmid

#80745

Purpose

Expression of human beta A1-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA1 (CRYBA1 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBB3

Plasmid

#80750

Purpose

Expression of human beta B3-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBB3 (CRYBB3 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Publication

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-L3F6H

Plasmid

#177765

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5'-GRIN2A

Plasmid

#244300

Purpose

5' AAVLINK plasmid for GRIN2A expression

Depositor

Zhonghua Lu

Publication

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

GRIN2A (GRIN2A Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG

Plasmid

#244301

Purpose

3' AAVLINK plasmid for GRIN2A expression

Depositor

Zhonghua Lu

Publication

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

GRIN2A (GRIN2A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYBB1

Plasmid

#217675

Purpose

For expression of SUMO tagged human beta B1 crystallin

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBB1 (CRYBB1 Human)

Tags

6XHis and Smt3

Expression

Bacterial

Promoter

T7

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYBA2

Plasmid

#80746

Purpose

Expression of human beta A2-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA2 (CRYBA2 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGB

Plasmid

#80751

Purpose

Expression of human gamma B-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGB (CRYGB Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGC

Plasmid

#80754

Purpose

Expression of human gamma C-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGC (CRYGC Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pESUMO-hRORg1

Plasmid

#170330

Purpose

Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 1 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPN094

Plasmid

#91621

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Publication

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Publication

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Publication

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Publication

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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shortVGLL3-GFP

Plasmid

#241148

Purpose

expresses short isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

shortVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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longVGLL3-GFP

Plasmid

#241149

Purpose

expresses long isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

longVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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