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DHFR-PP7-VP64_T2A_GFP

Plasmid

#86167

Purpose

Expresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-PP7-VP64_T2A_GFP

Use

CRISPR and Lentiviral

Tags

Destabilized domain (N-terminus version) of E.col…

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_miRFP703

Plasmid

#134985

Purpose

The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

miRFP703

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE1108

Plasmid

#202772

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_TagBFP

Plasmid

#134986

Purpose

The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

TagBFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pRG659_lexO-FseI_LexA-TF_LEU2MX

Plasmid

#154816

Purpose

Inducible expression of FseI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

FseI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSBbi-TIR1-tTA/Pur

Plasmid

#171683

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Inserts

tetracycline-controlled transactivator
Puromycin
EF-1a promoter

Expression

Mammalian

Promoter

EF-1a promoter and RPBSA promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRG645_lexO-AscI_LexA-TF_LEU2MX

Plasmid

#154813

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-Tetra-com-vector

Plasmid

#132553

Purpose

Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

Plasmid

#140221

Purpose

Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1 (Cas12a) N terminal half (406) with zipper

Expression

Mammalian

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

Plasmid

#140226

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg

Expression

Mammalian

Promoter

TRE3G

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x4

Plasmid

#58774

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge