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pAL10

Plasmid

#236006

Purpose

Backbone for assembling gRNA arrays with LacZ gene for blue white screening

Depositor

Carl de Boer

Article

Salaudeen et al Synth Biol (Oxf). 2025 Dec 18;11(1):ysaf

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-GFP-RGG

Plasmid

#242453

Purpose

Expresses the MBP-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

MBP-GFP-RGG

Tags

His and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GFP-RGG

Plasmid

#242454

Purpose

Expresses the GST-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

GST-GFP-RGG

Tags

GST and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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MOBE2

Plasmid

#219942

Purpose

Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-3'end_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1462

Plasmid

#218623

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1463

Plasmid

#218624

Purpose

Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1734

Plasmid

#218627

Purpose

Express amorphadiene synthase (ADS)

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

ADS

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1535

Plasmid

#218613

Purpose

Express Pex5

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1536

Plasmid

#218617

Purpose

Express Pex5 and Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1376

Plasmid

#218618

Purpose

Express Pex5, Pex3, Pex19, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1338

Plasmid

#218592

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB207

Plasmid

#218596

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS-His10

Plasmid

#223893

Purpose

4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-TwinStrep-His10

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_Avi-3C-TS

Plasmid

#223894

Purpose

4G-cloning donor for C-terminal AviTag-3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

AviTag-3C-TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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