bMS.346
(Bacterial strain #220588)

• Purpose: Increase editing efficiency of retron and oligo recombineering
• Depositing Lab: Seth Shipman
• Publication: Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. doi: 10.1038/s41589-021-00927-y. Epub 2021 Dec 23.

Backbone

• Vector backbone: n/a
• Vector type: This is a strain, not a plasmid

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): bMS.346
• Growth instructions: Tetracycline resistance is not necessary for growth
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: exoI- recJ- araB::T7RNAP-tetA
• Species: n/a

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Strain was validated by shotgun sequencing using Illumina Nextseq.

Supporting References:

Bhattarai-Kline, S., Lear, S.K., Fishman, C.B. et al. Recording gene expression order in DNA by CRISPR addition of retron barcodes. Nature 608, 217–225 (2022). https://doi.org/10.1038/s41586-022-04994-6.

González-Delgado A, Lopez SC, Rojas-Montero M, Fishman CB, Shipman SL. Simultaneous multi-site editing of individual genomes using retron arrays. bioRxiv [Preprint]. 2023 Jul 17:2023.07.17.549397. doi: 10.1101/2023.07.17.549397. PMID: 37503029; PMCID: PMC10370050.