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55_pAAV-ProA13-CatCh-GFP-WPRE

Plasmid

#125896

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA13

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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5_pAAV-ProA9-CatCh-GFP-WPRE

Plasmid

#125893

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProA9

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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105_pAAV-ProC16-CatCh-GFP-WPRE

Plasmid

#125951

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC16

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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214_pAAV-ProD22-CatCh-GFP-WPRE

Plasmid

#125998

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProD22

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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216_pAAV-ProD24-CatCh-GFP-WPRE

Plasmid

#126000

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProD24

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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153_pAAV-ProC31-CatCh-GFP-WPRE

Plasmid

#125966

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC31

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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167_pAAV-ProC34-CatCh-GFP-WPRE

Plasmid

#125968

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC34

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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13_pAAV-ProC71-CatCh-GFP-WPRE

Plasmid

#125976

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProC71

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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159_pAAV-ProD3-CatCh-GFP-WPRE

Plasmid

#125979

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Publication

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Promoter

ProD3

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

Plasmid

#113042

Purpose

AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

Plasmid

#113041

Purpose

AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-hSyn-PiGM-Ii (149, 535/81, eGFP)

Plasmid

#221610

Purpose

AAV encoding the 149 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

Depositor

John Lin

Insert

PiGM-Ii (149, eGFP)

Use

AAV

Mutation

RGS10(truncation after amino acid 149)

Promoter

Human Synapsin

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209663

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209659

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

GFAP

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -EGFP

Plasmid

#246994

Purpose

Can be used to generate AAV virus that will express EGFP from EF1α core promoter

Depositor

Ting Chen

Publication

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

EGFP

Use

AAV

Promoter

EF1α

Available since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-PGD2-1.1

Plasmid

#214764

Purpose

AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

Depositor

Yulong Li

Publication

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.1 sensor

Use

AAV

Promoter

EF-1-alpha promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40

Plasmid

#194689

Purpose

hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

Plasmid

#201685

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

Depositor

Erik Sontheimer

Publication

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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