We narrowed to 8,953 results for: plat

1. pUC19 gene 60 to gap

   Plasmid

   #49158

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap only

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Mutation

   Coding gap and downstream sequence have been del…

   Promoter

   T7

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC0940 (circRNA entry vector)

   Plasmid

   #189967

   Purpose

   Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. circRNA-synIRES-R25-NanoLuc

   Plasmid

   #188116

   Purpose

   Template plasmid to transcribe circular RNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. circRNA-synIRES-R25-mNeonGreen

   Plasmid

   #188115

   Purpose

   Template plasmid to transcribe circular RNA encoding mNeonGreen

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH136

   Plasmid

   #47554

   Purpose

   pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

   Depositor

   Ronald Davis

   Article

   Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

   Insert

   URA3
   

   Use

   Pcr template

   Promoter

   Native URA3 promoter, -243 to -1.

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Tags

   C-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-BC-EF1a-Puro

   Plasmid

   #127170

   Purpose

   PCR template for EF1a-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD547 ZF43x6-Compact_EYFP

   Plasmid

   #138861

   Purpose

   EYFP reporter vector for the ZF43x6-Compact promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF43x6-Compact

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. T7-CMVtrans-FFLuc-polyA

   Plasmid

   #101156

   Purpose

   Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

   Depositor

   Marcel Bruchez

   Article

   Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   T7 promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS41K-mCherry-sfGFP (pJJF001)

    Plasmid

    #173457

    Purpose

    C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-sfGFP
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid

    #109121

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ

    Depositor

    Allen Institute for Cell Science

    Insert

    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. AICSDP-58: NUP153-mEGFP

    Plasmid

    #114407

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human NUP153

    Depositor

    Allen Institute for Cell Science

    Insert

    NUP153 Homology Arms with mEGFP-linker (NUP153 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. AICSDP-26:ST6GAL1-mEGFP

    Plasmid

    #101786

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1

    Depositor

    Allen Institute for Cell Science

    Insert

    ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AICSDP-35:AAVS1-mEGFP

    Plasmid

    #91565

    Purpose

    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

    Depositor

    Allen Institute for Cell Science

    Insert

    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 7, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBS-GMR-eya(shRNA)

    Plasmid

    #157991

    Purpose

    pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

    Depositor

    Greg Beitel

    Article

    Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPD543 ZF21x6-Compact_EYFP

    Plasmid

    #138857

    Purpose

    EYFP reporter vector for the ZF21x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF21x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF21x6-Compact

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPD934 ZF37x6-Compact_EYFP

    Plasmid

    #138929

    Purpose

    EYFP reporter vector for the ZF37x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF37x6-Compact

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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