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We narrowed to 8,658 results for: plat

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Showing: 4601 - 4620 of 8658 results

  1. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  3. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid
    #155062
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155062-plasmid-map-sequence-id-308576

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  4. pHybr_r2a>mG3-srt-his

    Plasmid
    #124805
    Purpose
    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG3
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124805-plasmid-map-sequence-id-244310

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  8. ENDOD1-bio-His

    Plasmid
    #53155
    Purpose
    Expresses full-length ENDOD1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ENDOD1 (ENDOD1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53155-plasmid-map-sequence-id-82782

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  10. FLuc-C-NLuc

    Plasmid
    #234566
    Purpose
    Bacterially expressed FLuc-C-NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Firefly luciferase
    Tags
    NanoLuciferase-thrombin site-10xHis and T7 gene 1…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234566-plasmid-map-sequence-id-467872

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  12. qTAG-C-Solo-mStayGold-PEX3

    Plasmid
    #227304
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227304-plasmid-map-sequence-id-453636

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  13. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

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  14. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227321-plasmid-map-sequence-id-452094

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  15. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227287-plasmid-map-sequence-id-452057

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  18. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid
    #207794
    Purpose
    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207794-plasmid-map-sequence-id-416380

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  19. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid
    #207767
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207767-plasmid-map-sequence-id-413793

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  20. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid
    #207768
    Purpose
    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207768-plasmid-map-sequence-id-413803

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Showing: 4601 - 4620 of 8658 results