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pYPQ131-RZ-Cas12j2

Plasmid

#173927

Purpose

Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-RZ-Cas12j2

Plasmid

#173928

Purpose

Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ133-RZ-Cas12j2

Plasmid

#173929

Purpose

Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-RZ-Cas12j2

Plasmid

#173930

Purpose

Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#189782

Purpose

Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-RZ-Cas12j2

Plasmid

#189783

Purpose

Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRM214

Plasmid

#44527

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

K14R

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pRM204

Plasmid

#44528

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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Soat2_Luc

Plasmid

#177623

Purpose

Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

Depositor

Thomas Burris

Publication

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

human Soat2

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRM430

Plasmid

#44530

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-30

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pUK192

Plasmid

#44962

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

LEU2

Expression

Yeast

Mutation

HHTI-HHF1 is replaced with LEU2

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pRM415

Plasmid

#44526

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-15

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pRD661

Plasmid

#251525

Purpose

Overexpresses HTkC R45A in E. coli cells

Depositor

Remus Dame

Publication

Schwab et al bioRxiv 2025.12.06.692711; doi: 10.64898

Insert

HTkC R45A

Expression

Bacterial

Mutation

Changed Arginine 45 to Alanine

Promoter

T7 promotor

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c2

Plasmid

#242871

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Publication

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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