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pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRM214

Plasmid

#44527

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

K14R

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pRM204

Plasmid

#44528

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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Soat2_Luc

Plasmid

#177623

Purpose

Cloned human Soat2 promoter containing exon1 and 2 coupled to luciferase

Depositor

Thomas Burris

Publication

Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

Insert

human Soat2

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRM430

Plasmid

#44530

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-30

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pUK192

Plasmid

#44962

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

LEU2

Expression

Yeast

Mutation

HHTI-HHF1 is replaced with LEU2

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pRM415

Plasmid

#44526

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-15

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pRD661

Plasmid

#251525

Purpose

Overexpresses HTkC R45A in E. coli cells

Depositor

Remus Dame

Publication

Schwab et al bioRxiv 2025.12.06.692711; doi: 10.64898

Insert

HTkC R45A

Expression

Bacterial

Mutation

Changed Arginine 45 to Alanine

Promoter

T7 promotor

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGEL635

Plasmid

#189829

Purpose

The T-DNA backbone of Cas12j2 for genome editing in rice

Depositor

Yong Zhang

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Tags

ccdb

Promoter

ZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c2

Plasmid

#242871

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Publication

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
SM426_pHAGE-CAG-mCherry-T2A-puro-DR-As_crRNA-KAN-DR

Plasmid

#244226

Purpose

Vector for golden gate cloning AsCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-L7-3

Plasmid

#242870

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A425T)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, A425T

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 HuOpt Bxb1

Plasmid

#242869

Purpose

Mammalian expression of Bxb1 Integrase

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMOD_C2911

Plasmid

#161764

Purpose

MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

rolD:TREX2

Expression

Bacterial

Promoter

rolD

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Publication

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c15

Plasmid

#242873

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, E434G

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c10

Plasmid

#242872

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, E434G

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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