We narrowed to 14,923 results for: pET

1. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP1D1-Strep-II

   Plasmid

   #224209

   Purpose

   Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

   Depositor

   Anne Robinson

   Article

   Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

   Insert

   MSP1D1-Strep-II
   

   Use

   Tags

   Strep-II Tag

   Expression

   Bacterial

   Mutation

   Substitution of 7xHis tag found in pMSP1D1 for a …

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FepA

   Plasmid

   #26039

   Depositor

   Johann Deisenhofer

   Article

   Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

   Insert

   FepA (fepA E Coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pD-MSP1D1

   Plasmid

   #161607

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   D-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans were replaced b…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. His-Tev-UbE2D3

   Plasmid

   #186805

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE2D3 (UBE2D3 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUD-MSP1D1

    Plasmid

    #161608

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans and tyrosines w…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-ZB

    Plasmid

    #26107

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    His6 - Z-basic - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTSA-13

    Plasmid

    #17327

    Depositor

    Takeshi Sano

    Article

    Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ALIX-His(1-868, P801G)

    Plasmid

    #186808

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    ALIX-His (1-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge