We narrowed to 8,953 results for: plat

1. pUCmini-UGCG-tdTomato

   Plasmid

   #225879

   Purpose

   Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

   Depositor

   Yohei Sato

   Article

   Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRN3P_T3_BE4_IVT

   Plasmid

   #171762

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE4
   

   Use

   Vector for in-vitro transcription

   Promoter

   T3 promoter

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH3

   Plasmid

   #52528

   Purpose

   serves as PCR-template to generate HR donors for C-terminal GFP-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   eGFP and Blasticigin resistance cassette
   

   Use

   Blunt cloning kit

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN-PITCh-FAID

   Plasmid

   #127885

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-H3F-AID (PPP2CA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExpreS2-1

   Plasmid

   #175360

   Purpose

   ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1354

   Plasmid

   #69490

   Purpose

   Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

   Depositor

   Jordan Ward

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
   

   Use

   CRISPR; Template to generate cassettes for -media…

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2_RFP-60A

   Plasmid

   #200394

   Purpose

   Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   RFP
   

   Expression

   Mammalian

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD1029 ZF43x6-Compact(CMVMin)_EYFP

   Plasmid

   #138913

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(CMVmin)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF43x6-Compact(CMVmin)

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-144:CTCF-mEGFP

   Plasmid

   #168799

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

   Depositor

   Allen Institute for Cell Science

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pISH-NQO1

    Plasmid

    #136766

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Nqo1

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Nqo1 (Nqo1 Mouse)
    

    Use

    In situ probe

    Mutation

    ISH Probe insert, V184A- Please see depositor co…

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pES134

    Plasmid

    #127551

    Purpose

    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

    Depositor

    Emmanuele Severi, Gavin Thomas

    Article

    Wu et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    F3-flanked spectinomycin resistance cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH4

    Plasmid

    #52529

    Purpose

    serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    2x FLAG-tag
    

    Use

    Blunt cloning kit

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRN3P_T3_BE3_IVT

    Plasmid

    #177015

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE3
    

    Use

    CRISPR; Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLC-239

    Plasmid

    #73193

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-yhaV (yhaV )
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid

    #133340

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_gcrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIW1

    Plasmid

    #52530

    Purpose

    serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Twin-STREP-tag
    

    Use

    Blunt cloning kit

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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