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piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

Plasmid

#209078

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

3K mutant: E35K, E46K, E61K

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1

Plasmid

#79503

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt6

Plasmid

#35913

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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σ3-mCherry

Plasmid

#186580

Purpose

Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP3S1 (AP3S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso1E335A

Plasmid

#159587

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-hSV1_WT

Plasmid

#129483

Purpose

Mammalian expression of full-length human supervillin, isoform 1.

Depositor

Elizabeth Luna

Article

Smith et al Cytoskeleton (Hoboken). 2010 Jun;67(6):3

Insert

Supervillin (SVIL Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B WT

Plasmid

#123072

Purpose

Gateway entry clone encoding human MAP1LC3B wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Rat SERCA1a (pMT2)

Plasmid

#75182

Purpose

Mammalian expression of ATP2A1

Depositor

Jonathan Lytton

Article

Wu et al Am J Physiol. 1993 Feb;264(2 Pt 1):C333-

Insert

ATP2A1

Expression

Mammalian

Promoter

adenovirus major late promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2-puro

Plasmid

#44111

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
human p53-(1-393)-L344P

Plasmid

#24862

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Leucine 344 mutated to Proline

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT74

Plasmid

#204296

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN PLEKHM1-HA

Plasmid

#89300

Purpose

For expression of PLEKHM1 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

PLEKHM1 (PLEKHM1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN HA-GBRP

Plasmid

#89296

Purpose

For expression of the mammalian Atg8 protein GABARAP tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-pb6-flag

Plasmid

#86770

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 6 (PSMB6 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

unknown

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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