We narrowed to 5,525 results for: golden gate
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Plasmid#229872Purposegene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-Off[TetR-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1134
Plasmid#229870Purposegene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertcGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC
ExpressionWormAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
ExpressionWormAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-E566-GS-LOV2-VPR
Plasmid#231576PurposePotent light-deactivated CRISPRaDepositorInsertdSpyCas9-VPR with an AsLOV2 insertion behind E566
UseCRISPRExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_dCas9-AsLOV2x3-NLSx3
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
cpLOV2-Gal4
Plasmid#231569PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertcpLOV2-Gal4
ExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Zdk3-VP64
Plasmid#231571PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VP64
ExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-AtAGO4_gDNA
Plasmid#216838PurposeN-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoterDepositorAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p35S-FHA-SpAGO4a_gDNA
Plasmid#216841PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
TagsFlag-HAExpressionPlantPromoterCaMV 35SAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p35S-FHA-SpAGO4a_cDNA
Plasmid#216842PurposeN-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoterDepositorInsertARGONAUTE4
TagsFlag-HAExpressionPlantPromoterCaMV 35SAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV_Zdk3-VPR
Plasmid#231573PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertZdk3-VPR, CMV driven
ExpressionMammalianAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_REC8_cterm
Plasmid#222522PurposeCas9/sgRNA plasmid for targeting REC8DepositorPublicationInsertCas9, REC8 sgRNA (REC8 Synthetic, Human)
UseCRISPRAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMK83
Plasmid#226793PurposeIntegrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10BDepositorInsertslr0214 (slr0214 Synechocystis sp. PCC 6803)
ExpressionBacterialPromoterTetracycline resistance cassette promoter (consti…Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRDB_268
Plasmid#231112PurposeSpG-Cas9DepositorInsertCas9-SpG
UseCRISPR and LentiviralAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227925PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertYWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227926PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertYWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227914PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227918PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertDCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227920PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertHSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only