We narrowed to 14,926 results for: pET

1. pHIT342

   Plasmid

   #204514

   Purpose

   Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   N-terminal fragment of syp-1 (C. elegans)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIT320

   Plasmid

   #204517

   Purpose

   Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   Internal fragment of him-1 (C. elegans) (him-1 Nematode)
   

   Use

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLS.539

   Plasmid

   #184958

   Purpose

   Test effect of a1/a2 length on editing fabH using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   fabH donor G954A

   Promoter

   T7/lac

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRPF4.001.169

   Plasmid

   #137233

   Purpose

   Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

   Depositor

   Tara Davis

   Article

   Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

   Insert

   PRPF4 (PRPF4 Human)
   

   Use

   Tags

   (His)6-TEV

   Expression

   Bacterial

   Mutation

   001-169

   Promoter

   Available since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLS.543

   Plasmid

   #184960

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   murF donor G1359A

   Promoter

   T7/lac

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLS.545

   Plasmid

   #184962

   Purpose

   Test effect of a1/a2 length on editing priB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   priB donor G1069A

   Promoter

   T7/lac

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ppwd1.473.646.W601A

   Plasmid

   #137660

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Melissa Jurica, Tara Davis

   Article

   Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

   Insert

   ppwd1 (PPWD1 Human)
   

   Use

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   473-646-W601A

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD3cC

   Plasmid

   #86972

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

   Promoter

   T7 promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1G108CP20X

   Plasmid

   #85096

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS-3R112C/T180C

    Plasmid

    #85095

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Arginine 112 to Cysteine and Threonine 18…

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1G108C-GG

    Plasmid

    #85092

    Purpose

    Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Glycine 108 to Cysteine

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Danio rerio cryaa V62T

    Plasmid

    #47630

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Valine 62 to Threonine

    Promoter

    T7

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Danio rerio cryaa T147V

    Plasmid

    #47628

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed Theronine 147 to Valine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Danio rerio cryaa C143S

    Plasmid

    #47619

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 143 to Serine

    Promoter

    T7

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

    Plasmid

    #185713

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed W403 and F753 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p300 KAT LIC 1M

    Plasmid

    #233587

    Purpose

    Bacterial expression of p300 KAT enzyme

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300 KAT (EP300 Human)
    

    Use

    Tags

    6xHis MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSP1E3D1

    Plasmid

    #20066

    Purpose

    Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

    Insert

    MSP1E3D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeats of …

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge