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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,962 results for: pcDNA3.1+

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Showing: 4941 - 4960 of 6962 results

  6. LUC2 (L/Sec/deltaP)

    Plasmid
    #112145
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Publication
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U) and d…
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112145-plasmid-map-sequence-id-214904

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  9. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

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  10. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

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  11. [GFP][IRES][Bla]

    Plasmid
    #97194
    Purpose
    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
    Depositor
    Edward Boyden
    Publication
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta-Lactamase
    GFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97194-plasmid-map-sequence-id-197878

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  12. DsRed-CPI-17-wt

    Plasmid
    #100784
    Purpose
    Mammalian expression of Fluorescent CPI-17
    Depositor
    Joseph Gordon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CPI-17 (PPP1R14A Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100784-plasmid-map-sequence-id-193212

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  20. RF-118-UB-BRCA1

    Plasmid
    #78960
    Purpose
    Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells
    Depositor
    Richard Fahlman
    Publication
    Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRCA1 (BRCA1 Human)
    Tags
    3x Flag and ubiquitin
    Expression
    Mammalian
    Mutation
    delta 1-1155
    Promoter
    CMV
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78960-plasmid-map-sequence-id-173661

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Showing: 4941 - 4960 of 6962 results