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pAAV9-hSyn-iCytSnFR_ER

Plasmid

#177752

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCytSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

Plasmid

#162805

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA_IRES_Blasticidin

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDL128

Plasmid

#43912

Depositor

Stephen Elledge

Publication

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

SML domain mutated PPMLINQRT->AAAAAAAA, S59G, …

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pDL127

Plasmid

#43911

Depositor

Stephen Elledge

Publication

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Expression

Yeast

Mutation

HUG domain mutated VGMRIRQ->AAAAAAA, S59, Q66H

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCY 3120-04

Plasmid

#36238

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-13Myc-TRP1

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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VTA

Plasmid

#27805

Depositor

Steven Vogel

Publication

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Venus Traf Amber

Expression

Mammalian

Available since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pS60-ALFA-cpHalo179-ALFA-7xHis

Plasmid

#261833

Purpose

Expression of cpHalo179 protein with ALFA tag on both termini and 7xHis tag on the C-terminus

Depositor

Shaohe Wang

Publication

Wu et al bioRxiv 2026.08.18.745627

Insert

cpHalo179

Tags

7xHis and ALFA-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pS61-ALFA-cpWHalo179-ALFA-7xHis

Plasmid

#261834

Purpose

Expression of cpWHalo179 protein with ALFA tag on both termini and 7xHis tag on the C-terminus

Depositor

Shaohe Wang

Publication

Wu et al bioRxiv 2026.08.18.745627

Insert

WHalo179

Tags

7xHis and ALFA-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-pegRNA-acceptor-Puro

Plasmid

#253557

Purpose

pegRNA acceptor plasmid for sleeping beauty integration

Depositor

Ophir Shalem

Publication

Sanchez et al bioRxiv [Preprint]. 2024 Sep 17:2024.09.

Type

Empty backbone

Use

Unspecified

Available since

Sept. 2, 2026

Availability

Academic Institutions and Nonprofits only

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H2B-NovoTag494(L)

Plasmid

#255879

Purpose

Expression plasmid for NovoTag494(L), a NovoTag494 variant with long fluorescence lifetime, fused to H2B to localize histones

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoTag494(L)

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available since

Aug. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-Eevee-iAkt-FR

Plasmid

#252349

Purpose

Lentiviral transfer vector with Eevee-iAkt-FR.

Depositor

James Conway

Publication

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

Eevee-iAkt-FR

Use

Lentiviral

Promoter

CMV

Available since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-miRFP670nano3

Plasmid

#252339

Purpose

Transient expression of miRFP670nano3.

Depositor

James Conway

Publication

Tran et al Small Methods. 2026 Jul 12:e70828. doi:

Insert

miRFP670nano3

Tags

FLAG and V5; 6xHis

Expression

Mammalian

Promoter

EF1a

Available since

July 13, 2026

Availability

Academic Institutions and Nonprofits only

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pYS108

Plasmid

#255530

Purpose

Bacterial expression of W163(5HTP) variant of human γS-crystallin

Depositor

Rachel Martin

Publication

Seo et al Biophys Rep (N Y). 2026 Mar 11;6(1):1002

Insert

W163(5HTP) variant of human γS-crystallin (CRYGS Human)

Expression

Bacterial

Mutation

Tryptophan 163 changed to 5-hydroxytryptophan

Promoter

T7

Available since

June 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-VSG-228

Plasmid

#245117

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228

Use

Other

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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