We narrowed to 787 results for: pCMV-HA

1. pCMV5-AML1B

   Plasmid

   #12426

   Purpose

   pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

   Depositor

   Scott Hiebert

   Article

   Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

   Insert

   AML1B (RUNX1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

   Plasmid

   #185912

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

   Plasmid

   #185917

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV5 MEKK4alpha WT

   Plasmid

   #12187

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCMV5 MEKK4alpha KD

   Plasmid

   #12188

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Kinase dead: K1361M

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-APOBEC1-YTH

   Plasmid

   #131636

   Purpose

   Expresses APOBEC1 fused to the YTH domain

   Depositor

   Kate Meyer

   Article

   Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

   Insert

   APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-APOBEC1-YTHD422N

   Plasmid

   #194432

   Purpose

   APOBEC1 fused to YTHD422N

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHD422N (APOBEC1 Human)
   

   Use

   CRISPR

   Tags

   APOBEC1-YTHD422N-HA

   Expression

   Mammalian

   Mutation

   D422N mutation in the YTH domain to enhance m6A b…

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV5-AML1B

   Plasmid

   #12426

   Purpose

   pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

   Depositor

   Scott Hiebert

   Article

   Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

   Insert

   AML1B (RUNX1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid

    #223123

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid

    #228251

    Purpose

    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV5 MEKK3 WT

    Plasmid

    #12186

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK3 (Map3k3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCMV5 MEKK2 WT

    Plasmid

    #12182

    Depositor

    Gary Johnson

    Article

    Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

    Insert

    MEKK2 (Map3k2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-Mm.cargo(Cas9)

    Plasmid

    #174865

    Purpose

    Expresses mouse Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by MmPeg10 UTRs
    

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5B-TGFbeta receptor II wt

    Plasmid

    #11766

    Depositor

    Joan Massague, Jeff Wrana

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCMV-ADARcd-YTHD422N

    Plasmid

    #194402

    Purpose

    ADAR1 catalytic domain E488Q fused to YTHD422N

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    ADARcd fused to YTHD422N (ADAR Human)
    

    Tags

    ADARcd-YTHD422N-HA

    Expression

    Mammalian

    Mutation

    D422N mutation in the YTH domain to enhance m6A b…

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-ADARcd-YTHmut

    Plasmid

    #194431

    Purpose

    ADAR1 catalytic domain E488Q fused to YTHmut

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    ADARcd fused to YTHmut domain (ADAR Human)
    

    Tags

    ADARcd-YTHmut-HA

    Expression

    Mammalian

    Mutation

    m6A binding region of YTH domain is deleted

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-RosaR4 KKR mutations

    Plasmid

    #37199

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to RosaR4 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs R4, and SV40 NLS

    Expression

    Mammalian

    Mutation

    I538K, E490K, and H537R

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-RosaL6 ELD mutations

    Plasmid

    #37198

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to Rosa L6 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs L6, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q486E, I499L and N496D

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5 MEKK4alpha WT

    Plasmid

    #12187

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only