We narrowed to 597 results for: pFAST

1. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

   Plasmid

   #86843

   Purpose

   HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

   Depositor

   Thomas Cech

   Article

   Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

   Insert

   FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
   

   Use

   Tags

   FLAG-HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous TERT promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR660

   Plasmid

   #65216

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-8 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   P10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p130Cas Y15F

   Plasmid

   #186154

   Purpose

   For expressing recombinant p130Cas in insect cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Use

   Tags

   His6 (Tev)

   Expression

   Insect

   Mutation

   15Y residues mutated to Alanine

   Promoter

   polyhedrin

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFBD-Akt1(E17K)

   Plasmid

   #86563

   Purpose

   Expresses human full-length Akt1(E17K) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   E17K mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBD-Akt1(K14A)

   Plasmid

   #86562

   Purpose

   Expresses human full-length Akt1(K14A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   K14A mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBD-Akt13C(WT)

   Plasmid

   #86576

   Purpose

   Expresses cleavable human full-length Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB1.HMBP.PrS.PHF19

    Plasmid

    #125168

    Purpose

    Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Promoter

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-GFP(39TAG)

    Plasmid

    #217360

    Purpose

    Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    Enhanced green fluorescent protein
    

    Use

    Baculoviral

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Y39TAG

    Promoter

    CMV

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. R

    Plasmid

    #167170

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. S

    Plasmid

    #167172

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB-Flag-MyoD(QQQ)-E12

    Plasmid

    #26003

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

    Promoter

    Available since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

    Plasmid

    #226884

    Purpose

    Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

    Depositor

    Ning Zheng

    Article

    Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

    Insert

    NaVAb
    

    Use

    Tags

    MBP

    Expression

    Insect

    Mutation

    R4A, N49K, L109A, M116V

    Promoter

    Polyhedrin

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB-D3-ASK1

    Plasmid

    #226619

    Purpose

    Co-expression of D3 and ASK1 complex in insect cells

    Depositor

    Ning Zheng

    Article

    Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

    Inserts

    D3
    ASK1 (SKP1 Mustard Weed)
    

    Use

    Tags

    His tag followed with three copies of Msyb

    Expression

    Insect

    Mutation

    The flexible loop in D3 (Q478-D515) can be remove…

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. KIF13B

    Plasmid

    #163907

    Purpose

    Full length mouse KIF13B for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KIF13B 1-584

    Plasmid

    #163908

    Purpose

    Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    kif13b (Kif13b Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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