We narrowed to 14,926 results for: pET
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PDE4B-Sensor
Plasmid#208929Purposein vitro JNK-Sensor for luminescent detection of JNK-activityDepositorInsertPDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG
Plasmid#207631PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.DepositorInsertphotocaged SpyCatcher-cUnaG
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-Tev-ALIX-V (348-702)
Plasmid#189819PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).DepositorInsertGB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)
UseTagsGB1-His-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.544
Plasmid#184961PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
UseTagsExpressionBacterialMutationmurF donor G1359A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppih.001.177.W133A
Plasmid#137655PurposeExpresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppih (PPIH Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation001-177-W133APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_W403A_6xH
Plasmid#185712PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged W403 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK035
Plasmid#180827PurposeT7-inducible expression construct to produce SwGdmB (HY78_09235) in E. coliDepositorArticleInsertSwGdmB
UseTags6X HisExpressionBacterialMutationPromoterT7Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK036
Plasmid#180828PurposeT7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coliDepositorArticleInsertCnGdmA
UseTags6X HisExpressionBacterialMutationPromoterT7Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK066
Plasmid#180830PurposeT7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coliDepositorArticleInsertNaGdmA
UseTags6X HisExpressionBacterialMutationPromoterT7Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-5.3-GFP-HiBit
Plasmid#171014PurposeFor bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-5.3-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135G
Plasmid#160434PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to GlyDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Glycine; deleted the…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-Laforin
Plasmid#157989PurposeExpresses Laforin in E.coli cellDepositorInsertLaforin (EPM2A Human)
UseTagsHis TagExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC3R112C/T180C
Plasmid#85088PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450cam
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3PromoterT7 promoterAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180C-GG
Plasmid#85093PurposeHost vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationchanged Arginine 112 to Cysteine and Threonine 18…PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3R112C/T180CBM3
Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
