We narrowed to 15,165 results for: pET

1. pHIT517

   Plasmid

   #204518

   Purpose

   Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays

   Depositor

   Yuji Kohara

   Article

   Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

   Insert

   cec-4 (C. elegans)
   

   Tags

   6xHis, MBP

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Atg32 [200-341] 1B

   Plasmid

   #190431

   Purpose

   Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

   Depositor

   Michael Ragusa

   Article

   Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

   Insert

   Atg32
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLz54_polyPro_polar

   Plasmid

   #140847

   Purpose

   Same as pLz47 but with 20 polar amino acids instead of N*- peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with polar residues
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLz58_polyPro_CsoS2_CTP

   Plasmid

   #140850

   Purpose

   Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 C-terminal peptide
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz33_CsoS2_NTD_GFP

   Plasmid

   #140861

   Purpose

   His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD fused to GFP
   

   Tags

   Hexahistidine tag and Superfolder GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz55_polyPro_random_Npeptide

   Plasmid

   #140846

   Purpose

   Same as pLz47 but with randomized N*-peptide sequence

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with randomized CsoS2 N-peptide
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLz56_polyPro_CsoS2_MR_r1

   Plasmid

   #140848

   Purpose

   Same as pLz47 but with MR repeat 1 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 MR repeat 1
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLz57_polyPro_CsoS2_CTD_r8

   Plasmid

   #140849

   Purpose

   Same as pLz47 but with CTD repeat 8 instead of N*-peptide

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 CTD repeat 8
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWDY1226

   Plasmid

   #138593

   Purpose

   Expresses NatL2-R156A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Arginine 156 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWDY1222

    Plasmid

    #138592

    Purpose

    Expresses NatL2-K418A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Lysine 418 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZF-AA-GCN

    Plasmid

    #127479

    Purpose

    coding sequence for synthetic zinc finger ZF-AA-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-AA fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGS-3

    Plasmid

    #85094

    Purpose

    Host vector to fuse a gene at the N-terminus of PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC3

    Plasmid

    #85087

    Purpose

    Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    PCNA3 fused to P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Danio rerio cryaa

    Plasmid

    #46166

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cyprinodon variegatus cryaa

    Plasmid

    #47664

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaMCLC-His

    Plasmid

    #111292

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    (S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase
    

    Tags

    His tag, GGGSGGGSLEHHHHHH

    Expression

    Bacterial

    Availability

    Academic Institutions and Nonprofits only

    

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20. UbiGate Components (set1)

    Plasmid Kit

    #1000000144

    Purpose

    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays

    Depositor

    Marco Trujillo

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only