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We narrowed to 9,957 results for: pET

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Showing: 5001 - 5020 of 9957 results

  9. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Publication
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  10. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Publication
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  11. pET20b-His6-LONP1-Pore Loop 2 mutant (Y599A)

    Plasmid
    #247939
    Purpose
    Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form) Pore Loop 2 mutant (Y599A)
    Depositor
    Gabriel Lander
    Publication
    Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:
    Insert
    LONP1 (LONP1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    encodes aa 115-959, with Y599A
    Promoter
    T7
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247939-plasmid-map-sequence-id-491720

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  12. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Publication
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  13. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid
    #218973
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Publication
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Expression
    Mammalian
    Mutation
    mutant SRSF1 binding motif (AGTCTCCT)
    Promoter
    RSV LTR
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218973-plasmid-map-sequence-id-436111

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  14. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid
    #89287
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Publication
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 and Ar…
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89287-plasmid-map-sequence-id-175359

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  17. pET28a 6xHis-HaloTag-TEVc-4xtrTUBE

    Plasmid
    #254154
    Purpose
    Bacterial expression of 4xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al bioRxiv 2026.02.13.703496
    Insert
    4xtrTUBE (UBQLN1 Synthetic, Human)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254154-plasmid-map-sequence-id-511316

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  18. pET28a 6xHis-HaloTag-TEVc-4xtrTUBEmut

    Plasmid
    #254155
    Purpose
    Bacterial expression of binding-deficient 4xtrTUBEmut with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al bioRxiv 2026.02.13.703496
    Insert
    4xtrTUBEmut (UBQLN1 Synthetic, Human)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254155-plasmid-map-sequence-id-511310

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  19. pET28a 6xHis-HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254156
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al bioRxiv 2026.02.13.703496
    Insert
    6xtrTUBE (UBQLN1 Synthetic, Human)
    Tags
    6xHis, HaloTag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254156-plasmid-map-sequence-id-516711

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Showing: 5001 - 5020 of 9957 results