We narrowed to 14,926 results for: pET

1. H35R H43R SUMO1

   Plasmid

   #221063

   Purpose

   Expression of H35R H43R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H35R H43R double mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H35R, H43R

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. H43R SUMO1

   Plasmid

   #221057

   Purpose

   Expression of H43R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43R site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43R

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. H35R SUMO1

   Plasmid

   #221054

   Purpose

   Expression of H35R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H35R site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H35R

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. H43K SUMO1

   Plasmid

   #221058

   Purpose

   Expression of H43K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43K site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43K

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H43S SUMO1

   Plasmid

   #221059

   Purpose

   Expression of H43S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43S site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43S

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. H75K SUMO1

   Plasmid

   #221061

   Purpose

   Expression of H75K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75K site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75K

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. H75S SUMO1

   Plasmid

   #221062

   Purpose

   Expression of H75S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75S site mutation on SUMO 1 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75S

   Promoter

   T7

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MZB224

   Plasmid

   #226998

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Use

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   T31N

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSD118A

   Plasmid

   #218140

   Purpose

   Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD0_ZF2-3
   

   Use

   Tags

   AviTag-6xHis-MBP-TEV-(GGGGS)2

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RC(14-3-3ε)-LgBit

    Plasmid

    #208926

    Purpose

    Recognition domain for in vitro RSK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    14-3-3ε (YWHAQ Human)
    

    Use

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB1-His-TEV-A7 (2-180)

    Plasmid

    #190066

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-His-TEV-A7-Strep (2-180)

    Plasmid

    #190067

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV and Strep

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GB1-PRD(703-868, P801G)

    Plasmid

    #180029

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTarget_PS_VA

    Plasmid

    #186454

    Purpose

    pTarget containing protospacer for FnCas12a targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTarget_priming_VA

    Plasmid

    #186455

    Purpose

    pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTarget_PS_VB

    Plasmid

    #186456

    Purpose

    pTarget containing protospacer for AaCas12b targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEUK028

    Plasmid

    #180826

    Purpose

    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEUK037

    Plasmid

    #180829

    Purpose

    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RGD-I27-CC

    Plasmid

    #85393

    Purpose

    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p1G108CFa

    Plasmid

    #85089

    Purpose

    Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C in PCNA1, A74G/C773S/C810S in FAD domain of…

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge