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pRS416-PTEF1-MCP-5M-ECM33_intron

Plasmid

#127600

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-5P-ECM33_intron

Plasmid

#127599

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron with MS2 hairpins inserted near 5′ end of intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-ECM33_intron

Plasmid

#127598

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

Depositor

David Bartel

Publication

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

ECM33 intron

Expression

Yeast

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMM0626

Plasmid

#129001

Purpose

pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAL- S. pombe TSC1 (1-431)

Plasmid

#132616

Purpose

expresses S. pombe TSC1 (1-431) in E. coli

Depositor

Wenqing Xu

Publication

Sun et al Nat Commun. 2013;4:2135. doi: 10.1038/nc

Insert

TSC1 (1-431)

Expression

Bacterial

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0627

Plasmid

#129002

Purpose

pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0557

Plasmid

#128991

Purpose

LEU2 integration vector for Zif268DBD-CRY2

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2-tADH1-URA3-Ura3 5' homology

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0558

Plasmid

#128992

Purpose

LEU2 integration vector for Zif268DBD-CRY2PHR

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

Ura3 3' homology-pTDH3-SV40NLS-ZiF268-CRY2PHR-tADH1-URA3-Ura3 5' homology

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0317

Plasmid

#128979

Purpose

Encodes Zif268DBD-CRY2 under pscADH1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0320

Plasmid

#128980

Purpose

Encodes Zif268DBD-CRY2PHR under pscADH1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0521

Plasmid

#128984

Purpose

Expression plasmid for ZIF268DBD-L3-CRY2

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-Zif268DBD-CRY2 (L3) scTRP1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0620

Plasmid

#128995

Purpose

pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0628

Plasmid

#129003

Purpose

pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMV45

Plasmid

#128885

Purpose

Position 3 module- 3SV40NLS (3x NLS from SV40)

Depositor

Gilles Charvin

Publication

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

SV40 NLS

Use

Synthetic Biology

Expression

Yeast

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JC034

Plasmid

#104947

Purpose

Methanol inducing hGH, and estrogen inducing G-CSF

Depositor

Timothy Lu

Publication

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

hGH and G-CSF

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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JC135

Plasmid

#104946

Purpose

Methanol inducing HSA, and estrogen inducing hGH

Depositor

Timothy Lu

Publication

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA and hGH

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-10X-tRNA

Plasmid

#70123

Purpose

Gateway DONR vector with cassette for expression of 10 intronless S. cerevisiae tRNAs

Depositor

Jay Hesselberth

Publication

Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

Insert

10X-tRNA block

Promoter

None – SUP4 pol III promoter provided by insert

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pTD125 GFP:POP1

Plasmid

#71387

Purpose

localization of yeast RNase POP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Publication

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

POP1 (POP1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

ACT1

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
pRS415-Cox15-13xMyc

Plasmid

#61721

Purpose

yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

Depositor

Oleh Khalimonchuk

Publication

Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

Insert

Cox15 (COX15 Budding Yeast)

Tags

13xMyc

Expression

Yeast

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only