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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon-CGHL

Plasmid

#221658

Purpose

Rubicon mutated at four residues in the RH domain to disrupt Rab7 binding, tagged at the N-terminus with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Mutation

C912G, C915G, H920L, C923G

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME-B-catenin-S33Y-FLAG (JDW 419)

Plasmid

#242565

Purpose

Gateway middle entry clone containing dominant active B-catenin (S33Y beta catenin)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

S33Y B-catenin (CTNNB1 Human)

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-AkaLuc-P2A-Proinsulin

Plasmid

#241776

Purpose

Tested on HeLa for bioluminescence, and used for production of AAV G12-Akaluc-P2A-Proinsulin for mice.

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

AkaLuc-P2A-Proinsulin

Use

AAV

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX1-1-175-EGFP-Hygro

Plasmid

#238093

Purpose

Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX1 (DTX1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-175 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX4-1-166-EGFP-Hygro

Plasmid

#238095

Purpose

Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX4 (DTX4 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-CAG_G-PTEN

Plasmid

#227438

Purpose

An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor (Pten Rat)

Use

AAV

Tags

CD-sREACh and mEGFP

Expression

Mammalian

Mutation

R14G

Promoter

pCAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NIR-FbLAG30

Plasmid

#220748

Purpose

NIR-FbLAG30 expression in bacteria.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Bacterial

Mutation

For bacterial cytoplasmic expression, the pelB si…

Promoter

T7

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1 BRCT domain deletion

Plasmid

#207110

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 with a deletion of the BRCT domain in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1 BRCT domain deletion

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169-Halo HRD

Plasmid

#207083

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBImut-T2A-caMEK

Plasmid

#199579

Purpose

Express tKiMBImut(AA) and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator (mutant)
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pHcRed-CLCa

Plasmid

#196911

Purpose

visualization of clathrin-coated vesicle in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

Clathrin-Coated light chain

Tags

HcRed

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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