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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-cytoB5RR

Plasmid

#182577

Purpose

Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-cytoB5RR

Tags

LifeAct and cytoB5RR

Expression

Mammalian

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-cytoB5RR

Plasmid

#182577

Purpose

Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-cytoB5RR

Tags

LifeAct and cytoB5RR

Expression

Mammalian

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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myc-Raptor

Plasmid

#1859

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Raptor (RPTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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myc-Raptor

Plasmid

#1859

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Raptor (RPTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Article

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

P438

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_P438-mEOS3.2_GentaR

Plasmid

#173894

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the P438 promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

P438

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge