We narrowed to 15,186 results for: pET

1. E67R SUMO1

   Plasmid

   #221064

   Purpose

   Expression of E67R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   E67R site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E67R

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. H35R H43R SUMO1

   Plasmid

   #221063

   Purpose

   Expression of H35R H43R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H35R H43R double mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H35R, H43R

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. H43R SUMO1

   Plasmid

   #221057

   Purpose

   Expression of H43R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43R site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43R

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. H35R SUMO1

   Plasmid

   #221054

   Purpose

   Expression of H35R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H35R site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H35R

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. H43K SUMO1

   Plasmid

   #221058

   Purpose

   Expression of H43K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43K site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43K

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. H43S SUMO1

   Plasmid

   #221059

   Purpose

   Expression of H43S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H43S site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H43S

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. H75K SUMO1

   Plasmid

   #221061

   Purpose

   Expression of H75K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75K site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75K

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. H75S SUMO1

   Plasmid

   #221062

   Purpose

   Expression of H75S SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   H75S site mutation on SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   H75S

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MZB224

   Plasmid

   #226998

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   Arf1 (ARF1 Human)
   

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   T31N

   Promoter

   T7

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSD118A

    Plasmid

    #218140

    Purpose

    Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD0_ZF2-3
    

    Tags

    AviTag-6xHis-MBP-TEV-(GGGGS)2

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. RC(14-3-3ε)-LgBit

    Plasmid

    #208926

    Purpose

    Recognition domain for in vitro RSK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    14-3-3ε (YWHAQ Human)
    

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-His-TEV-A7 (2-180)

    Plasmid

    #190066

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Tags

    GB1-His-TEV

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1-His-TEV-A7-Strep (2-180)

    Plasmid

    #190067

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Tags

    GB1-His-TEV and Strep

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-PRD(703-868, P801G)

    Plasmid

    #180029

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
    

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTarget_PS_VA

    Plasmid

    #186454

    Purpose

    pTarget containing protospacer for FnCas12a targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTarget_priming_VA

    Plasmid

    #186455

    Purpose

    pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTarget_PS_VB

    Plasmid

    #186456

    Purpose

    pTarget containing protospacer for AaCas12b targeting

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEUK028

    Plasmid

    #180826

    Purpose

    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    SwGdmA (SWIT_RS16490 Sphingomonas wittichii RW1, Synthetic)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEUK037

    Plasmid

    #180829

    Purpose

    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RGD-I27-CC

    Plasmid

    #85393

    Purpose

    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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