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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27,344 results for: grna

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Showing: 5261 - 5280 of 27344 results

  2. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    70019-plasmid-map-sequence-id-124558

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  9. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

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  10. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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  11. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

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  15. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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  20. pBFC0694

    Plasmid
    #186693
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_lacZ_α_1 gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186693-plasmid-map-sequence-id-371383

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Showing: 5261 - 5280 of 27344 results