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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 5361 - 5380 of 25285 results

  3. pKm-HABE

    Plasmid
    #170332
    Purpose
    a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti
    Depositor
    Deqiang Duanmu
    Publication
    Wang et al Front Microbiol. 2021 Jun 18;12:686008.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rhizobium codon-optimized ABEs
    Use
    CRISPR
    Mutation
    Cas9n-D10A
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    170332-plasmid-map-sequence-id-341070

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  11. pJAT32

    Plasmid
    #204297
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204297-plasmid-map-sequence-id-405508

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  13. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  14. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    170185-plasmid-map-sequence-id-333571

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  18. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68417-plasmid-map-sequence-id-120195

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  20. dSp-VP64-p65(100-261)-RTA(125-190)

    Plasmid
    #99673
    Purpose
    Expresses dSp Cas9 fused to combination of truncated activation domains
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Tags
    VP64-p65(101-261)-RTA(125-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    99673-plasmid-map-sequence-id-192082

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Showing: 5361 - 5380 of 25285 results