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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 5381 - 5400 of 6562 results

  1. pEMS1665

    Plasmid
    #29289
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple193 (SLC6A3 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Sept. 1, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29289-plasmid-map-sequence-id-13704

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  9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  12. HaloTag-LacI-KRAB

    Plasmid
    #232638
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor
    Depositor
    Karsten Rippe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag-LacI-KRAB
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232638-plasmid-map-sequence-id-462856

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  13. 3xFLAG-dCas9/pMSCVneo

    Plasmid
    #134982
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134982-plasmid-map-sequence-id-430627

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  15. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  17. pEMS1408

    Plasmid
    #29205
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple201 (SLC6A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29205-plasmid-map-sequence-id-13620

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  18. pEMS1482

    Plasmid
    #29235
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Elizabeth Simpson
    Publication
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple12 (AVP Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Oct. 20, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29235-plasmid-map-sequence-id-13650

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  19. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  20. LLP779_αGCN4-VP64-CO

    Plasmid
    #211772
    Purpose
    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection
    Depositor
    Ryan Lister
    Publication
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    aGCN4-VP64
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect VP64 e…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211772-plasmid-map-sequence-id-417389

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Showing: 5381 - 5400 of 6562 results