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Addgene

pEMS1136
(Plasmid #29056)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29056 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEMS1307
  • Backbone size w/o insert (bp) 16482
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple198
  • Alt name
    SLC6A4
  • Alt name
    solute carrier family 6, member 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2826
  • Entrez Gene
    SLC6A4 (a.k.a. 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, SERT1, hSERT)
  • Tag / Fusion Protein
    • EGFP-NLS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site FseI (unknown if destroyed)
  • 3′ cloning site AscI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1136 was a gift from Elizabeth Simpson (Addgene plasmid # 29056 ; http://n2t.net/addgene:29056 ; RRID:Addgene_29056)
  • For your References section:

    New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914