We narrowed to 17,399 results for: grna

1. pSB700 lenti gRNA Hygro_zhang2.0

   Plasmid

   #167915

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB700 lenti gRNA EYFP_zhang2.0

   Plasmid

   #167914

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Bmpr2 gRNA resistant

   Plasmid

   #163408

   Purpose

   Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   gRNA-targeting sequences are substituted with nuc…

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Bmpr2ΔEC gRNA resistant

   Plasmid

   #163413

   Purpose

   Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The extracellular domain (27-150 aa) is deleted. …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Bmpr2ΔTail gRNA resistant

   Plasmid

   #163412

   Purpose

   Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The C-terminal tail domain (530-1038 aa) is delet…

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-U63-tgRNA-Gal80

   Plasmid

   #169030

   Purpose

   gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

   Depositor

   Chun Han

   Article

   Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVD1 tRNA-gRNA E4-En-1 (GB2243)

   Plasmid

   #160565

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E4-En-1 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1026L=U6-3-gRNA#1[Pol-γ35]

   Plasmid

   #159674

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#1[Pol-γ35]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1026M=U6-3-gRNA#2[Pol-γ35]

   Plasmid

   #159675

   Purpose

   Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   U6-3-gRNA#2[Pol-γ35]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNTI763 p(Z)-mCherry + UBC9 sgRNA

    Plasmid

    #164918

    Purpose

    CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(Z)-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P(Z), synthetic promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

    Plasmid

    #159290

    Purpose

    Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT6A (ID. A8) (KAT6A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

    Plasmid

    #149589

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W

    Plasmid

    #159289

    Purpose

    Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    guide RNA targeting KAT6A (ID. A7) (KAT6A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge