We narrowed to 16,162 results for: grna

1. pX330-MM002-ZFP143-C-terminal-sgRNA

   Plasmid

   #212706

   Purpose

   Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

   Depositor

   Elzo de Wit

   Insert

   ZFP143 C-terminal sgRNA (Zfp143 Mouse)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223323

   Purpose

   Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Insert

   SpCas9 and sgRNA-T2 (cas9 )
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

   Plasmid

   #203443

   Purpose

   Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

   Plasmid

   #203447

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

   Plasmid

   #203451

   Purpose

   Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-non targeting (NT) sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Codon optimised using GenScript tool

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

   Plasmid

   #227800

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 2 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

   Plasmid

   #227801

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 3 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

   Plasmid

   #209922

   Purpose

   A knock-out vector for the dog CDH1

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   A gRNA targeting the dog CDH1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HEK4-53bp-tag-insertion-pegRNA

   Plasmid

   #227673

   Purpose

   PegRNA for 53 bp insertion

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   SpCas9 sgRNA scaffold (EPHA3 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

    Plasmid

    #206178

    Purpose

    Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Cre
    

    Use

    AAV

    Promoter

    cTnT

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-U6.3-sgRNA-empty-GFP

    Plasmid

    #221843

    Purpose

    empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Insert

    EGFP
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Expression

    Mammalian

    Promoter

    CAGC

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-U6.3-sgRNA-DNM1L -GFP

    Plasmid

    #221845

    Purpose

    Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Expression

    Mammalian

    Promoter

    CAGC and U6.3 chick

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-U6.3-sgRNA-MFN1-GFP

    Plasmid

    #221846

    Purpose

    Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Expression

    Mammalian

    Promoter

    CAGC and U6.3 chick

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX552-EF1a-DIO-mCherry_Dicer gRNA

    Plasmid

    #215279

    Purpose

    Expresses Dicer gRNA in a Cre dependent mcherry vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Dicer control gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX552-EF1a-DIO-mCherry_DicerTTT gRNA

    Plasmid

    #215280

    Purpose

    Expresses Dicer Control gRNA in a Cre dependent mcherry vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Dicer control gRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

    Plasmid

    #215281

    Purpose

    Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX552-hSyn-DIO-GCaMP8f_Grin1TTT gRNA

    Plasmid

    #215282

    Purpose

    Expresses Grin1 Control gRNA in a Cre dependent GCaMP8f vector

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Grin1 control gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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