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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 CDK9 HA (P#635)

Plasmid

#14640

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 (CDK9 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a

Plasmid

#25030

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP1a (DCP1A Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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HA Seh1L pRK5

Plasmid

#46331

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Seh1 (SEH1L Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab5a-c-Myc

Plasmid

#79801

Purpose

Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO V5 DNAJB4

Plasmid

#19526

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 wt

Plasmid

#107104

Purpose

Lentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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CPSF5

Plasmid

#155480

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF5 (NUDT21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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hRad51(S208E-A209D)–Cas9(D10A)

Plasmid

#125561

Purpose

Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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HA WDR24 pRK5

Plasmid

#46335

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pBV-Luc wt MBS1-4

Plasmid

#16564

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

c-MYC binding sites (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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Daxx/pEGFP

Plasmid

#119021

Purpose

Express GFP-Daxx in mamalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

death-domain associated protein (Daxx) (DAXX Human)

Tags

GFP

Expression

Mammalian

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV Myc IRES GFP

Plasmid

#18770

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

Myc (Myc Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
413 pSG5L HA RB

Plasmid

#10720

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (RB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-Parkin

Plasmid

#17612

Depositor

Ted Dawson

Article

Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

Insert

Parkin (PRKN Human)

Tags

Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-RFP

Plasmid

#35395

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
RFP

Use

Retroviral

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only