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pST_DrHD_dom3_k5_0 (pBS0758)

Plasmid

#185242

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k5_0

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_7 (pBS0755)

Plasmid

#185241

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_7

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DrHD_dom3_k3_1 (pBS0754)

Plasmid

#185240

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DrHD_dom3_k3_1

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_AdAPOA4-44 (pBS0745)

Plasmid

#185236

Purpose

For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdAPOA4-44

Expression

Mammalian

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Bighead S-FC

Plasmid

#180147

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Publication

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Tags

FC

Expression

Mammalian

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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iDianiSnFR

Plasmid

#177741

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iDianiSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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iCytSnFR

Plasmid

#177738

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCytSnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_BrEt_SnFR

Plasmid

#177740

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_BrEt_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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iCyt_F_SnFR

Plasmid

#177739

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

iCyt_F_SnFR

Tags

HA, His, and Myc

Expression

Bacterial

Promoter

T7

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTD73

Plasmid

#172897

Purpose

Intestinal promoter ges-1P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTD81

Plasmid

#172902

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1 (TIR1 Mustard Weed)

Use

CRISPR

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJRK109

Plasmid

#173733

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Publication

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

CRY2(olig)

Use

Other

Mutation

E490G

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Publication

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Tags

HA

Expression

Mammalian

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK3_IRS

Plasmid

#109885

Purpose

Protein expression and purification of DOK3_IRS

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK3_IRS (DOK3 Human)

Expression

Bacterial

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_IRS1_IRS

Plasmid

#109892

Purpose

Protein expression and purification of IRS1_IRS

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

IRS1_IRS (IRS1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ECH1_ECH_1

Plasmid

#109860

Purpose

Protein expression and purification of ECH1_ECH_1

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ECH1_ECH_1 (ECH1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPRED2_WH1

Plasmid

#109825

Purpose

Protein expression and purification of SPRED2_WH1

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPRED2_WH1 (SPRED2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMyo-3::oxCerulean-oxVenus::Lgg-1

Plasmid

#68044

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagy

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pMyo-3::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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