We narrowed to 17,340 results for: emb

1. pDEST/LIfeAct-mCherry-N1

   Plasmid

   #40908

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   LifeAct
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   No stop codon to create c-terminal fusion

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2699-CMV-Nme2Cas9-E932R

   Plasmid

   #228720

   Purpose

   Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-E932R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2696-CMV-Nme2SmuCas9-D56R

   Plasmid

   #228717

   Purpose

   Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-D56R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS2697-CMV-Nme2SmuCas9-E932R

   Plasmid

   #228718

   Purpose

   Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-E932R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS2698-CMV-Nme2Cas9-D56R

   Plasmid

   #228719

   Purpose

   Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-D56R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKL10-mRuby-iATPSnFR1.0 bb118

   Plasmid

   #158980

   Purpose

   Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   mruby-iATPSNfR1.0
   

   Tags

   mruby3

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#102557

   Promoter

   Bba_J23118 (sigma 70)

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYS16

   Plasmid

   #202082

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   CT mNG-T2A-PuroR Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Promoter

   None

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-Rab8A[Q67L]

   Plasmid

   #24900

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[Q67L]
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Glutamine 67 to Leucine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-Rab8A[T22N]

   Plasmid

   #24899

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   hRab8A[T22N]
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 22 to Asparagine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-C1-AKT-PH

    Plasmid

    #21218

    Depositor

    Tobias Meyer

    Article

    Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

    Insert

    AKT-PH
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of AKT. (Lab plasmid WC 0002).

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG79-attL1-pegRNA-attL2

    Plasmid

    #213050

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. IL-2 promoter luciferase

    Plasmid

    #12194

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-2 promoter (Il2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMU-PERr

    Plasmid

    #61202

    Purpose

    Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    mCherry-SKL
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYS29

    Plasmid

    #202086

    Purpose

    Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    NT HygR-T2A-Halo Minicircle Parent
    

    Use

    CRISPR

    Tags

    HaloTag

    Promoter

    None

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. EasyFusion T2A-H2B-miRFP703

    Plasmid

    #113097

    Purpose

    General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-miRFP703
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOPO mKate2

    Plasmid

    #68441

    Purpose

    TOPO construct expressing mKate2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mKate2
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-miniTurbo-Puro

    Plasmid

    #207725

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1-300(HAT-)

    Plasmid

    #23254

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Expression

    Mammalian

    Mutation

    H1415A E1423A Y1424A L1428S Y1430A H1434A

    Available Since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP-GFP-Sec61β

    Plasmid

    #172442

    Purpose

    Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Protein transport protein Sec61 subunit beta
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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