We narrowed to 9,454 results for: Pol

Showing: 5781 - 5800 of 9454 results

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pCR 2.1-TOPO Fzd6HA

Plasmid

#208363

Purpose

Extracellular HA-tagged Fzd6 (mouse)

Depositor

Maureen Cetera, Danelle Devenport

Article

Cetera et al Development. 2023 Aug 25:dev.202078. doi

Insert

Fzd6 (Fzd6 Mouse)

Use

Cloning vector

Tags

HA

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 360-684

Plasmid

#205055

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Expression

Bacterial

Mutation

Lacks N terminal amino acid from 1-359

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C-b(E184Q)-HA

Plasmid

#209264

Purpose

Mammalian expression of ATP11C isoform b mutant E184Q

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Use

Retroviral

Tags

HA

Mutation

E184Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C-c-HA

Plasmid

#209222

Purpose

Mammalian expression of ATP11C isoform c

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform c (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C-b-HA

Plasmid

#209221

Purpose

Mammalian expression of ATP11C isoform b

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bctd-L7G-Cas9n-UGI-NLS

Plasmid

#198886

Purpose

Express A3Bctd-L7G BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Mutation

Loop 7 of A3G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bctd-D314E-Cas9n-UGI-NLS

Plasmid

#198887

Purpose

Express A3Bctd-D314E BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

NLS

Expression

Mammalian

Mutation

D314E

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eA3Ai-Cas9n-UGI-NLS

Plasmid

#207165

Purpose

Express A3A with an N57G point mutation and an intron in a BE3-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI--NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eA3Ai-max

Plasmid

#207166

Purpose

Express A3A with an N57G point mutation and an intron in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)

Tags

Cas9-UGI-UGI-NLS and NLS

Expression

Mammalian

Mutation

N57G

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-CI

Plasmid

#196852

Purpose

Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

Plasmid

#197969

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V, delta(1616-1666)

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

Plasmid

#197970

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V, delta(1616-1666)

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed -7e

Plasmid

#191765

Purpose

Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of negatively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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H1.M10.luc

Plasmid

#193706

Purpose

Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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