We narrowed to 6,252 results for: nls

1. pPB-CAG-Brainbow3.2-PGK-PuroTK

   Plasmid

   #159928

   Purpose

   Brainbow transgenesis

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

   Inserts

   NFPnls
   mOrange2
   EGFP
   mKate2
   

   Expression

   Mammalian

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK1_022

   Plasmid

   #123684

   Purpose

   Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-A4 insulator
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUbi-MAD7

   Plasmid

   #170133

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al J Genet Genomics. 2021 May 3. pii: S1673

   Insert

   MAD7(ErCas12a)
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   maize Ubiquitin-1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Promoter

   HAM34 and RPL41

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK527

   Plasmid

   #110605

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pRRL-GFPGO2(NGG)-PGK-Neo

   Plasmid

   #136891

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2(NGG)
   

   Use

   Lentiviral

   Tags

   2xNLS

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sABE v3.22 Lenti_2

    Plasmid

    #209556

    Purpose

    pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
    

    Expression

    Mammalian

    Promoter

    pU6, pCMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. attB53-Pac-attB53

    Plasmid

    #183610

    Purpose

    RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Pac
    

    Use

    Rmce

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-LbCas12a

    Plasmid

    #213743

    Purpose

    For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDA125

    Plasmid

    #74249

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Expression

    Bacterial and Yeast

    Promoter

    pSTL1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK1_014

    Plasmid

    #123677

    Purpose

    Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS'-FRT
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. iPE-WT-Cas9

    Plasmid

    #239379

    Purpose

    For inverse prime editor using WTCas9 in HEK294T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

    Insert

    Cas9, M-MLV RT
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRO211

    Plasmid

    #233830

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pTDH3-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-mNeonGreen-tENO2
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRO261

    Plasmid

    #233831

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRPL18B-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-mNeonGreen-tENO2
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRO262

    Plasmid

    #233832

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRNR2-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRNR2-mNeonGreen-tENO2
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRO263

    Plasmid

    #233833

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRAD27-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRAD27-mNeonGreen-tENO2
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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