We narrowed to 8,829 results for: sgRNA

1. pLentiCMVblast_SLC38A2_N82A

   Plasmid

   #156181

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   CMV

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer20_SLC38A2_N82A

   Plasmid

   #156183

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEE

   Plasmid

   #159746

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HK1 gRNA (BRDN0001145157)

   Plasmid

   #77643

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human HK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   HK1 (HK1, HOOK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HK1 gRNA (BRDN0001147564)

   Plasmid

   #77644

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human HK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   HK1 (HK1, HOOK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HEK4-53bp-tag-insertion-pegRNA

   Plasmid

   #227673

   Purpose

   PegRNA for 53 bp insertion

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   SpCas9 sgRNA scaffold (EPHA3 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI AscI MR1 res

   Plasmid

   #214752

   Purpose

   expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

   Depositor

   David Lewinsohn

   Article

   Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

   Insert

   MHC class I-related protein 1 (MR1 Human)
   

   Tags

   IRES eGFP

   Expression

   Mammalian

   Mutation

   silent mutations to prevent editing by CRISPR/Cas…

   Promoter

   CMV

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209069

   Purpose

   Entry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

   Insert

   sgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase
   

   Use

   Gateway vector to be used for lr reaction

   Promoter

   U6

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR223-CMV-Cre-U6-sgNotch1#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209068

   Purpose

   Entry vector that encodes sgRNAs against mouse Notch1, Rb1, Trp53, Rbl2, and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Hong et al Cancer Res. 2022 Jan 15;82(2):248-263. d

   Insert

   sgRNAs targeting Notch1, Rb1, Trp53, Rbl2 and Cre recombinase
   

   Use

   Gateway vector to be used for lr reaction

   Promoter

   U6

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKHH030_sgCtrl#2

    Plasmid

    #174143

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid

    #173169

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    Synonymous mutations at sgRNA sites, amino acids …

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid

    #173167

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Mutation

    Synonymous mutations at sgRNA sites, G2A, S6A

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. px330-Kmt2b-4

    Plasmid

    #162538

    Purpose

    sgRNA targeting Kmt2b

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2b (Kmt2b Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. px330-Kmt2c-1

    Plasmid

    #162531

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px330-Kmt2c-2

    Plasmid

    #162532

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px330-Kmt2c-3

    Plasmid

    #162533

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px330-Kmt2b-3

    Plasmid

    #162537

    Purpose

    sgRNA targeting Kmt2b

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2b (Kmt2b Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px330-Kmt2b-2

    Plasmid

    #162536

    Purpose

    sgRNA targeting Kmt2b

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2b (Kmt2b Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px330-Kmt2b-1

    Plasmid

    #162535

    Purpose

    sgRNA targeting Kmt2b

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2b (Kmt2b Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px330-Kmt2c-4

    Plasmid

    #162534

    Purpose

    sgRNA targeting Kmt2c

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    sgRNA targeting Kmt2c (Kmt2c Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge