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pGS-BacD-23003

Plasmid

#109183

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacD-23005

Plasmid

#109185

Purpose

Plasmid donor for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV KI;Sptbn1 Donor;smFP-V5

Plasmid

#240309

Purpose

KI:Sptbn1

Depositor

Yuki Ogawa

Insert

KI gRNA for Sptbn4

Use

AAV

Mutation

NA

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-PAF1

Plasmid

#231728

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Publication

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

PAF1 (PAF1 Human)

Expression

Insect

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-GST-TEV

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS

Plasmid

#213393

Purpose

uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL1160

Plasmid

#196025

Purpose

Expression/purification of 6xHis-TEV-MlaD

Depositor

Gira Bhabha, Damian Ekiert

Publication

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Publication

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pACEBac1-Q22MS1-2xStrep

Plasmid

#170314

Purpose

Plasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag II

Depositor

Andrew Carter

Publication

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1

Tags

Strep-Tag II

Expression

Insect

Promoter

Polyhedrin

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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PHY55-mouse U6-sgLMNA

Plasmid

#164045

Purpose

U6-driven sgRNA targeting LMNA

Depositor

Baohui Chen

Publication

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

LMNA targeting sgRNA

Use

CRISPR

Promoter

mouse U6

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pROS15_MATz

Plasmid

#166100

Purpose

Expression of dual gRNA targeting the MAT locus

Depositor

Rebecca Burgess

Insert

MAT Z1

Use

CRISPR

Expression

Yeast

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1305

Plasmid

#155143

Purpose

Expresses MlaFB, His tag on MlaF N-term

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

(6xHis-TEV-MlaF)-MlaB

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1307

Plasmid

#155145

Purpose

Expresses MlaFB, His tag on MlaB N-term

Depositor

Gira Bhabha, Damian Ekiert

Publication

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-(6xHis-TEV-MlaB)

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1996

Plasmid

#139900

Purpose

Expression plasmid for LetB PLL4 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Publication

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta459-360)-6xHis

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2002

Plasmid

#139887

Purpose

Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Publication

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta161-514)-6xHis

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2001

Plasmid

#139886

Purpose

Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Publication

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta161-634)-6xHis

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z32

Plasmid

#120075

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z10

Plasmid

#120053

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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