Addgene: CRISPR-Cas12a-assisted PCR tagging of mammalian genes.

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Michael Knop
Fueller et al

CRISPR-Cas12a-assisted PCR tagging of mammalian genes.
Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M
J Cell Biol. 2020 Jun 1;219(6). pii: 151766. doi: 10.1083/jcb.201910210. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
119980	pMaCTag-01	Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP	Loading...
119981	pMaCTag-02	Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP	Loading...
119982	pMaCTag-03	Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3	Loading...
119983	pMaCTag-04	Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1	Loading...
119984	pMaCTag-05	Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP	Loading...
119985	pMaCTag-06	Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP	Loading...
119986	pMaCTag-33	Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG	Loading...
119987	pMaCTag-08	Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3	Loading...
119988	pMaCTag-09	Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus	Loading...
119989	pMaCTag-10	Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk	Loading...
119990	pMaCTag-11	Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i	Loading...
119991	pMaCTag-12	Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet	Loading...
119992	pMaCTag-13	Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry	Loading...
119993	pMaCTag-14	Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5	Loading...
119994	pMaCTag-15	Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670	Loading...
119995	pMaCTag-16	Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3	Loading...
119996	pMaCTag-17	Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b	Loading...
119997	pMaCTag-18	Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5	Loading...
119998	pMaCTag-34	Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG	Loading...
119999	pMaCTag-20	Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag	Loading...
120000	pMaCTag-21	Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag	Loading...
120001	pMaCTag-22	Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP	Loading...
120002	pMaCTag-23	Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo	Loading...
120003	pMaCTag-24	Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP	Loading...
120004	pMaCTag-25	Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID	Loading...
120005	pMaCTag-26	Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover	Loading...
120006	pMaCTag-27	Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA	Loading...
120007	pMaCTag-28	Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA	Loading...
120008	pMaCTag-29	Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II	Loading...
120009	pMaCTag-30	Template for C-terminal PCR tagging of mammalian genes (without selection) with VSV-G	Loading...
120010	pMaCTag-31	Template for C-terminal PCR tagging of mammalian genes (without selection) with V5	Loading...
120011	pMaCTag-32	Template for C-terminal PCR tagging of mammalian genes (without selection) with S-Tag	Loading...
120012	pMaCTag-P01	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP	Loading...
120013	pMaCTag-P02	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP	Loading...
120014	pMaCTag-P03	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3	Loading...
120015	pMaCTag-P04	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1	Loading...
120016	pMaCTag-P05	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP	Loading...
120017	pMaCTag-P06	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP	Loading...
120018	pMaCTag-P33	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG	Loading...
120019	pMaCTag-P08	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3	Loading...
120020	pMaCTag-P09	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus	Loading...
120021	pMaCTag-P10	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk	Loading...
120022	pMaCTag-P11	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i	Loading...
120023	pMaCTag-P12	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet	Loading...
120024	pMaCTag-P13	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry	Loading...
120025	pMaCTag-P14	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5	Loading...
120026	pMaCTag-P15	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670	Loading...
120027	pMaCTag-P16	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3	Loading...
120028	pMaCTag-P17	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b	Loading...
120029	pMaCTag-P18	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5	Loading...
120030	pMaCTag-P34	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG	Loading...
120031	pMaCTag-P20	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag	Loading...
120032	pMaCTag-P21	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag	Loading...
120033	pMaCTag-P22	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP	Loading...
120034	pMaCTag-P23	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo	Loading...
120035	pMaCTag-P24	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP	Loading...
120036	pMaCTag-P25	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID	Loading...
120037	pMaCTag-P26	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover	Loading...
120038	pMaCTag-P27	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA	Loading...
120039	pMaCTag-P28	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA	Loading...
120040	pMaCTag-P29	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II	Loading...
120041	pMaCTag-P30	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G	Loading...
120042	pMaCTag-P31	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5	Loading...
120043	pMaCTag-P32	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag	Loading...
120044	pMaCTag-Z01	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP	Loading...
120045	pMaCTag-Z02	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP	Loading...
120046	pMaCTag-Z03	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3	Loading...
120047	pMaCTag-Z04	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1	Loading...
120048	pMaCTag-Z05	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP	Loading...
120049	pMaCTag-Z06	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP	Loading...
120050	pMaCTag-Z33	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG	Loading...
120051	pMaCTag-Z08	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3	Loading...
120052	pMaCTag-Z09	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus	Loading...
120053	pMaCTag-Z10	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk	Loading...
120054	pMaCTag-Z11	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i	Loading...
120055	pMaCTag-Z12	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet	Loading...
120056	pMaCTag-Z13	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry	Loading...
120057	pMaCTag-Z14	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5	Loading...
120058	pMaCTag-Z15	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670	Loading...
120059	pMaCTag-Z16	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3	Loading...
120060	pMaCTag-Z17	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b	Loading...
120061	pMaCTag-Z18	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5	Loading...
120062	pMaCTag-Z34	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG	Loading...
120063	pMaCTag-Z20	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag	Loading...
120064	pMaCTag-Z21	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag	Loading...
120065	pMaCTag-Z22	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP	Loading...
120066	pMaCTag-Z23	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo	Loading...
120067	pMaCTag-Z24	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP	Loading...
120068	pMaCTag-Z25	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID	Loading...
120069	pMaCTag-Z26	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover	Loading...
120070	pMaCTag-Z27	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA	Loading...
120071	pMaCTag-Z28	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA	Loading...
120072	pMaCTag-Z29	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II	Loading...
120073	pMaCTag-Z30	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G	Loading...
120074	pMaCTag-Z31	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5	Loading...
120075	pMaCTag-Z32	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag	Loading...
124786	pMaCTag-07	Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen	Loading...
124787	pMaCTag-19	Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen	Loading...
124788	pMaCTag-P07	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen	Loading...
124789	pMaCTag-P19	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen	Loading...
124790	pMaCTag-Z07	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen	Loading...
124791	pMaCTag-Z19	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen	Loading...

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Recombinant Antibodies from Article