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CRISPR-Cas12a-assisted PCR tagging of mammalian genes.
Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M
J Cell Biol. 2020 Jun 1;219(6). pii: 151766. doi: 10.1083/jcb.201910210.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119980pMaCTag-01Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP
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119981pMaCTag-02Template for C-terminal PCR tagging of mammalian genes (without selection) with TagBFP
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119982pMaCTag-03Template for C-terminal PCR tagging of mammalian genes (without selection) with moxCerulean3
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119983pMaCTag-04Template for C-terminal PCR tagging of mammalian genes (without selection) with mTFP1
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119984pMaCTag-05Template for C-terminal PCR tagging of mammalian genes (without selection) with eGFP
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119985pMaCTag-06Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP
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119986pMaCTag-33Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG
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119987pMaCTag-08Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3
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119988pMaCTag-09Template for C-terminal PCR tagging of mammalian genes (without selection) with mVenus
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119989pMaCTag-10Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk
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119990pMaCTag-11Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i
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119991pMaCTag-12Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet
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119992pMaCTag-13Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry
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119993pMaCTag-14Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5
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119994pMaCTag-15Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670
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119995pMaCTag-16Template for C-terminal PCR tagging of mammalian genes (without selection) with Dronpa3
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119996pMaCTag-17Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b
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119997pMaCTag-18Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5
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119998pMaCTag-34Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG
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119999pMaCTag-20Template for C-terminal PCR tagging of mammalian genes (without selection) with 10xSuntag
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120000pMaCTag-21Template for C-terminal PCR tagging of mammalian genes (without selection) with 24xSunTag
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120001pMaCTag-22Template for C-terminal PCR tagging of mammalian genes (without selection) with SNAP
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120002pMaCTag-23Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo
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120003pMaCTag-24Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP
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120004pMaCTag-25Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID
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120005pMaCTag-26Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover
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120006pMaCTag-27Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA
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120007pMaCTag-28Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA
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120008pMaCTag-29Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II
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120009pMaCTag-30Template for C-terminal PCR tagging of mammalian genes (without selection) with VSV-G
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120010pMaCTag-31Template for C-terminal PCR tagging of mammalian genes (without selection) with V5
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120011pMaCTag-32Template for C-terminal PCR tagging of mammalian genes (without selection) with S-Tag
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120012pMaCTag-P01Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP
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120013pMaCTag-P02Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP
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120014pMaCTag-P03Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3
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120015pMaCTag-P04Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1
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120016pMaCTag-P05Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP
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120017pMaCTag-P06Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP
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120018pMaCTag-P33Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG
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120019pMaCTag-P08Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3
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120020pMaCTag-P09Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mVenus
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120021pMaCTag-P10Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk
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120022pMaCTag-P11Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i
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120023pMaCTag-P12Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet
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120024pMaCTag-P13Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry
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120025pMaCTag-P14Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5
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120026pMaCTag-P15Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670
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120027pMaCTag-P16Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Dronpa3
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120028pMaCTag-P17Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b
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120029pMaCTag-P18Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5
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120030pMaCTag-P34Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG
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120031pMaCTag-P20Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag
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120032pMaCTag-P21Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag
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120033pMaCTag-P22Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP
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120034pMaCTag-P23Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo
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120035pMaCTag-P24Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP
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120036pMaCTag-P25Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID
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120037pMaCTag-P26Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover
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120038pMaCTag-P27Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA
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120039pMaCTag-P28Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA
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120040pMaCTag-P29Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II
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120041pMaCTag-P30Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G
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120042pMaCTag-P31Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5
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120043pMaCTag-P32Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag
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120044pMaCTag-Z01Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxBFP
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120045pMaCTag-Z02Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP
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120046pMaCTag-Z03Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with moxCerulean3
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120047pMaCTag-Z04Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1
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120048pMaCTag-Z05Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP
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120049pMaCTag-Z06Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP
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120050pMaCTag-Z33Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG
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120051pMaCTag-Z08Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3
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120052pMaCTag-Z09Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus
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120053pMaCTag-Z10Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKOk
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120054pMaCTag-Z11Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i
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120055pMaCTag-Z12Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet
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120056pMaCTag-Z13Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry
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120057pMaCTag-Z14Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5
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120058pMaCTag-Z15Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670
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120059pMaCTag-Z16Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3
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120060pMaCTag-Z17Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b
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120061pMaCTag-Z18Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5
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120062pMaCTag-Z34Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG
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120063pMaCTag-Z20Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag
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120064pMaCTag-Z21Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTag
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120065pMaCTag-Z22Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP
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120066pMaCTag-Z23Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo
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120067pMaCTag-Z24Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TAP
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120068pMaCTag-Z25Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID
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120069pMaCTag-Z26Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover
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120070pMaCTag-Z27Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 1xHA
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120071pMaCTag-Z28Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xHA
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120072pMaCTag-Z29Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II
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120073pMaCTag-Z30Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G
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120074pMaCTag-Z31Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5
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120075pMaCTag-Z32Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag
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124786pMaCTag-07Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen
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124787pMaCTag-19Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen
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124788pMaCTag-P07Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen
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124789pMaCTag-P19Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen
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124790pMaCTag-Z07Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen
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124791pMaCTag-Z19Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen
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